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维生素D₃可以通过免疫调节有效地和快速地清除大鱼 ranavirus

Pengxu Wang¹, Xingchen Huo², Fengxia Zhao²

¹Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.

Fish & shellfish immunology

|November 10, 2023

概括

维生素D3 (VD3) 通过降低病毒载量和改善鱼类免疫力,有效治疗大口鱼角病毒 (LMBV). 补充VD3显示了在水产养殖中对LMBV疾病的显著治疗潜力.

科学领域:

水生动物的健康状况.
鱼类免疫学鱼类免疫学
拉纳病毒研究研究

背景情况:

大口的拉纳病毒 (LMBV) 导致大口的显著死亡率.
对于LMBV感染,治疗选择有限.
维生素D3 (VD3) 在免疫功能中起着至关重要的作用.

研究的目的:

评估食维生素D3 (VD3) 补充剂作为治疗大嘴鱼中LMBV的疗效.
评估不同的VD3剂量对LMBV复制,免疫基因表达和鱼类整体健康的影响.

主要方法:

鱼类的食补充了不同剂量的VD3.
使用定量逆转录PCR (qRT-PCR) 和半-qPCR来测量LMBV负载和免疫基因表达 (IL-1β,IFN-γ,Mx,IgM).
分析了血清生物化学指数 (TSOD,TAOC,C3,总蛋白质) 和组织病理.

主要成果:

饮食VD3在15000 IU/Kg和20000 IU/Kg显著抑制和清除LMBV在14天内.
补充VD3可提高关键免疫相关基因的调节,增强抗氧化剂和免疫活动.
较高的VD3剂量 (15000 IU和20000 IU) 以剂量依赖的方式将组织损伤降到最低.

结论:

饮食VD3是一种有前途的治疗剂,可以对抗LMBV,减少病毒复制和减轻组织损伤.
补充VD3有效地提高了免疫反应,并改善了LMBV感染鱼的血清生物化学概况.
VD3为水产养殖中LMBV疾病提供了可行的替代治疗策略,提高了鱼类健康和农场生产率.

关键词:

免疫保护免疫保护大嘴鱼 (Micropterus salmoides) 是一种鱼.大口拉纳病毒 (LMBV)病毒清除时间维生素D ((3)) (VD ((3))) 是一种维生素.

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