通过调节Pseudomonas fluorescens生物膜的形成
Lei Yuan1, Yanhe Zhang2, Zizhuo Mi2
1School of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China; Key Laboratory of Dairy Science (Northeast Agricultural University), Ministry of Education, Harbin 150030, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China.
Journal of dairy science
|November 10, 2023
概括
(Ca2+) 增强了乳制品Pseudomonas fluorescens中的生物膜的形成. 这项研究发现Ca2+增加了生物膜容量和多糖生产,同时改变了与定数感应和运动性相关的基因表达.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物技术是生物技术.
背景情况:
- 生物膜的形成受到环境因素的影响,特别是双价离子.
- 乳制品Pseudomonas光体在乳制品行业具有重要意义,其影响生物膜形成过程.
研究的目的:
- 为了研究 (Ca2+) 对乳制品Pseudomonas fluorescens分离物的生物膜形成的影响.
- 通过转录基因分析阐明Ca2+在生物膜发育中的调节机制.
主要方法:
- 在不同度的Ca2+下评估生长,蜂群运动,生物膜形成和细胞外聚合物物质的产生.
- 使用扫描电子显微镜 (SEM) 和共聚焦激光扫描显微镜 (CLSM) 进行生物膜结构分析.
- 执行RNA测序来分析对Ca2+补充剂的反应中的基因表达变化.
主要成果:
- 补充5和10mMCa2+增加了生物膜形成能力和多糖体的生产,尽管减少了群体运动.
- SEM和CLSM证实了Ca2+处理的P. fluorescens P.F4中增强的生物膜复杂性,生物量和覆盖范围.
- RNA测序揭示了P. fluorescens P.F4中的显著差异性基因表达,影响诸如定数感应和鞭毛组装等途径.
结论:
- (Ca2+) 作为乳制品Pseudomonas fluorescens中的生物膜形成的显著增强剂.
- 2+通过基因表达的调节影响生物膜发育,影响运动性和矩阵生产.
- 结果表明,Ca2+的潜在应用是为了控制或促进乳制品行业中的P. fluorescens生物膜.
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