长小RNA76113的目标是循环核酸门离子通道5,以抑制大米的疾病耐药性
Liyu Zheng1,2, Yiyang Yu1,2, Ying Zheng1,2
1Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China.
Plant physiology
|November 10, 2023
概括
长小RNAs调节大米的免疫力. 一种特定的长小RNA (lsiR76113) 针对OsCNGC5,控制的流入,通过调节PAMP触发的免疫力来增强对Magnaporthe oryzae的抵抗力.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物病理学 植物病理学
- 在RNA生物学,RNA生物学.
背景情况:
- 小RNA在植物免疫力中至关重要.
- 长小RNAs (25-40 nt) 在单胞菌病耐药性中的功能在很大程度上仍未被探索.
研究的目的:
- 为了研究长小RNA在抗米病的作用.
- 阐明 lsiR76113 影响大米免疫力对抗 Magnaporthe oryzae 的分子机制.
主要方法:
- 在大米中识别和描述一种新的长小RNA (lsiR76113).
- 米爆发病原体Magnaporthe oryzae感染的分析.
- 基因表达分析和突变生成 (cngc5突变系).
- 原生体短暂表达试验用于研究OsCNGC5功能.
- 评估PAMP触发的免疫反应 (PTI),包括活性氧物种 (ROS) 爆发和基因激活蛋白激酶 (MAPK) 级联酸化.
主要成果:
- lsiR76113在M. oryzae感染时被下调,并准OsCNGC5.5.
- OsCNGC5通过促进Ca2+流入,增加细胞溶液Ca2+度来增强大米对M. oryzae的耐药性.
- 外源的Ca2+应用通过影响ROS产量,提高了米的抗性.
- cngc5突变对M. oryzae的敏感性降低,lsiR76113的淘汰增加了耐药性.
- OsCNGC5对于PAMP诱导的信号传递至关重要,因为cngc5突变体表现出减弱的PTI反应.
结论:
- OsCNGC5通过调节Ca2+流入和PTI,在防爆米饭中发挥着至关重要的作用.
- lsiR76113介导的Ca2+流入的调节是PTI和大米疾病耐药性的关键机制.
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