来自人类癌症生物样本的高质量的基因组规模DNA甲基化测序数据生成的协议
Euan J Rodger1, Peter A Stockwell1, Suzan Almomani1
1Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.
STAR protocols
|November 11, 2023
概括
这项研究详细介绍了在多种癌症样本中高质量的DNA甲基化测序的协议. 该方法可以为癌症研究和其他疾病提供全面的甲基形状分析.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
- 癌症生物学 癌症生物学
背景情况:
- 异常的DNA甲基化是癌症的标志.
- 了解癌症表观遗传学需要强大的基因组规模甲基化数据.
研究的目的:
- 为生成高质量的基因组规模DNA甲基化测序数据提出一个标准化的协议.
- 为了在各种人类癌症生物标本中实现全面的甲基组概况.
主要方法:
- 针对不同组织类型的DNA提取优化.
- 减少表示双硫酸盐测序 (RRBS) 方法.
- 生物信息管道用于数据处理,质量控制和分析.
主要成果:
- 从细胞系,新鲜冷和FFPE组织中成功生成高质量的DNA甲基化数据.
- 一个适用于不同样本类型和生物体的综合性协议.
- 建立下游数据整合和分析的框架.
结论:
- 提出的协议有助于在癌症中进行稳健的甲基形状分析.
- 这种方法适用于研究其他人类疾病和非人类生物.
- 标准化表观基因组数据生成对于推进癌症研究至关重要.
相关概念视频
00:58Genomic DNA in Eukaryotes
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
03:00Next-generation Sequencing
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.