从未充分利用的棕色藻 (Padina tetrastromatica) 通过亚临界水解来提取福科伊丹后的过程优化和水解盐的表征
Nidhi Hans1, Divyang Solanki2, Tanya Nagpal3
1Supercritical Fluid Extraction Laboratory, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi 110016, India.
Journal of environmental management
|November 11, 2023
概括
亚临界水解有效地从Padina tetrastromatica中恢复蛋白质和生物活性,为营养品和药品提供可持续的来源. 这种绿色技术产生具有抗氧化和酶抑制特性的有价值化合物,对健康应用有益.
科学领域:
- 海洋生物技术 海洋生物技术
- 绿色化学 绿色化学
- 生物化学工程 生物化学工程
背景情况:
- 对于蛋白质,和氨基酸的宏生物质的需求日益增长.
- 需要从藻类中采用可持续和高效的蛋白质提取方法.
- 对藻类衍生化合物的生物和功能性质感兴趣.
研究的目的:
- 为了研究在Padina tetrastromatica中提取蛋白质的亚临界水解的使用.
- 优化最大限度地恢复蛋白质的条件,并识别生物活性.
- 评估产生的水解盐的潜在应用.
主要方法:
- 在100-220°C的温度下使用亚临界水进行Padina tetrastromatica的水解.
- 优化温度,时间和生物质与水的比率 (1:50 w/v) 在4.0 MPa.
- 分析蛋白质含量,自由氨基酸,水解程度,分子量,氨基酸概况,和黄.
主要成果:
- 在最佳条件下 (10分钟的220°C) 产生了最大的蛋白质 (127.2 mg g-1),自由氨基酸 (58.4 mg g-1),以及高水解度 (58.8%).
- 化剂含有45%的必需氨基酸,包括甲氨酸,单氨酸和氨酸.
- 显著的抗氧化活性和抑制乙胆酶 (IC50:17.9 mg mL-1) 和α-氨酶 (IC50:16.0%).
结论:
- 环保的亚临界水解有效地从P. tetrastromatica中恢复79%的蛋白质.
- 化物是一种丰富的生物活性的来源,具有潜在的营养,制药和化品应用.
- 这种方法提供了一种可持续的方法,用于利用大藻生物质生产高价值产品.
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