参与者收集的家用灰尘用于评估城市住宅中的微生物和半挥发性有机化合物
D Jarma1, J P Maestre1, J Sanchez2
1Department of Civil, Architectural, and Environmental Engineering, The University of Texas at Austin, TX, USA.
The Science of the total environment
|November 11, 2023
概括
参与者收集的尘埃样本是室内微生物群和化学物质暴露研究的可行选择. 分析显示,与研究人员收集的样本相比,微生物多样性和化学化合物检测相似,尽管样本质量较低.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 室内环境中存在复杂的微生物群落和化学污染物.
- 评估室内暴露通常依赖于研究人员收集的样本,这可能是昂贵和耗时的.
- 参与者收集的样本 (PCS) 为大型室内环境研究提供了一个潜在的可扩展的替代方案.
研究的目的:
- 评估使用参与者收集的尘埃样本 (PCS) 来评估室内环境暴露的可行性.
- 为了比较PCS的微生物和化学成分与研究人员收集的样本 (RCS).
- 确定PCS是否可以准确地表示室内微生物群和半挥发性有机化合物 (SVOC) 档案.
主要方法:
- 研究人员和参与者从43个美国城市住宅收集了尘埃样本.
- 使用16S和ITS测序分析了细菌和真菌群落.
- 用GC-MS.测量了27种半挥发性有机化合物 (SVOC) 的度.
主要成果:
- 在PCS中的微生物α和β多样性与RCS没有显著不同.
- 人类相关的细菌种群和户外相关的真菌种群在两个样本类型中占主导地位.
- 通常检测到SVOCs,PCS和RCS中有机酸盐和酸盐的频率相似.
结论:
- 参与者收集的尘埃样本是室内微生物组研究的可行和可行的选择.
- PCS可以为RCS提供可比的微生物社区数据,支持大规模的住宅研究.
- 虽然SVOC分析存在挑战,但PCS显示了全面室内环境暴露评估的潜力.
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