丰富的光性紫色细菌对H2生物转化成单细胞蛋白质的潜力
María Del Rosario Rodero1, Jose Antonio Magdalena2, Jean-Philippe Steyer3
1INRAE, Univ Montpellier, LBE, 102 Avenue des Etangs, 11100 Narbonne, France; Institute of Sustainable Processes, University of Valladolid, Dr. Mergelina, s/n, 47011 Valladolid, Spain; Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina, s/n, 47011 Valladolid, Spain.
The Science of the total environment
|November 11, 2023
概括
这项研究表明,富含光紫色细菌 (PPB) 可以有效地将和二氧化碳转化为单细胞蛋白 (SCP). 生产的SCP具有高蛋白质含量和适合动物料的氨基酸特征.
科学领域:
- 微生物学和生物技术
- 可持续的资源管理可持续的资源管理
- 食品科学与技术 食品科学与技术
背景情况:
- 单细胞蛋白 (SCP) 提供了替代蛋白质来源,通过利用废物来源的资源与循环经济原则保持一致.
- 气态基质,如 (H2) 和二氧化碳 (CO2),由于其固有的无菌性,有利于SCP生产,产生无病原体的生物质.
- 光紫色细菌 (PPB) 是一个有前途的微生物群体,用于生物转化过程,利用光作为能源.
研究的目的:
- 评估一种富含光性紫色细菌 (PPB) 联盟的有效性,用于使用H2和CO2生产单细胞蛋白 (SCP).
- 确定PPB生长动力学和生物质产量的最佳环境条件 (pH,温度,光强).
- 评估产生的SCP的蛋白质含量,氨基酸概况和适合动物料应用的SCP.
主要方法:
- 进行了批量测试,以调查pH值 (6.0-8.5),温度 (15-50°C) 和光强度 (0-50 W·m-2) 对PPB生长的影响.
- 生物质和蛋白质产量是基于消耗的H2和CO2的量化.
- 使用机械模型估计吸收率,并分析生物质成分的蛋白质和氨基酸含量.
主要成果:
- 对PPB的最佳生长条件是在pH7,25°C,光强度超过30W·m-2的条件下确定.
- 实现了高生物质和蛋白质产量 (~1g COD生物质·g CODH2消耗-1和3.9-4.4g蛋白质·g H2-1),是气态基质报告的最高产量之一.
- 生产的SCP生物量含有50%以上的蛋白质,具有有利的氨基酸概况,PPB以*Rhodobacter*和*Rhodopseudomonas*物种为主,保持了较高的相对丰度 (>80%).
结论:
- 丰富的PPB培养显示出有效的H2生物转化到高质量的SCP的显著潜力.
- 由于PPB能够利用光作为唯一的能量来源,这使得PPB能够完全利用H2进行生长,从而获得高产量.
- 生产的SCP适用于动物料,有助于可持续的蛋白质生产和循环经济框架内的废物回收利用.
相关概念视频
Anoxygenic Phototrophic Bacteria
34
Anoxygenic phototrophic bacteria are a diverse group of microorganisms that perform photosynthesis without producing oxygen. They primarily include purple sulfur bacteria, purple nonsulfur bacteria, green sulfur bacteria, and green nonsulfur bacteria. These bacteria are classified into the Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Chlorobi, and Chloroflexi lineages, each with distinct physiological and ecological adaptations.Purple sulfur bacteria belong to the...
34
Anoxygenic Photosynthesis
24
Anoxygenic photosynthesis is a phototrophic process that captures light energy to drive carbon fixation without producing molecular oxygen. Unlike oxygenic photosynthesis, which utilizes water as an electron donor and releases oxygen, anoxygenic phototrophs use alternative electron donors such as hydrogen sulfide (H₂S), elemental sulfur (S⁰), or thiosulfate (S₂O₃²⁻). This process is carried out by diverse groups of bacteria, including purple bacteria, green...
24
Bioremediation
18.6K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
18.6K
Microbial Nutrition
38
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
38
Green Algae
26
Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...
26
Bacterial Phylum Cyanobacteria
27
Cyanobacteria are a diverse group of oxygenic, phototrophic bacteria that played a pivotal role in converting Earth’s atmosphere from anoxic to oxygen-rich billions of years ago. They exhibit remarkable morphological diversity, ranging from unicellular forms to filamentous types, with cell sizes varying between 0.5 μm and 100 μm. Cyanobacteria are classified into five groups: Chroococcales (unicellular, dividing by binary fission), Pleurocapsales (unicellular, dividing by...
27


