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Updated: Jul 11, 2025

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses
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Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

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一个多重实时RT-PCR系统,同时诊断与猪呼吸道疾病相关的16种病原体

Yusuke Goto1, Kazuhiro Fukunari1, Shigekatsu Tada1

  • 1Central Iwate Prefectural Livestock Health and Hygiene Center, Takizawa, Iwate 020-0605, Japan.

Journal of applied microbiology
|November 11, 2023
PubMed
概括

一个新的多重实时RT-qPCR系统可以同时检测16种猪呼吸道疾病 (SRD) 病原体. 这种快速诊断工具有助于对猪进行早期感染控制和治疗.

关键词:
pleuropneumoniae 的阿克提诺细菌 (Actinobacillus) 是一个波德泰拉 (Bordetella) 支气管感染病.血类虫 (Haemophilus parasuisis) 是一种有毒的病毒.菌质低肺炎症是如何发生的这种植物是Pasteurella multocida.沙门氏菌spp.p.p. 的情况.这是一种Streptococcus suis.艾奥斯基病病毒病毒的存在.经典猪瘟病毒 经典猪瘟病毒诊断 诊断 诊断 诊断 诊断 诊断多重实时RT-PCR多重反应猪猪病毒 2 猪猪病毒 2猪肉病毒3猪肉病毒3猪细胞巨型病毒病毒猪生殖和呼吸系统综合征病毒欧洲基因型猪生殖和呼吸系统综合征病毒北美基因型猪呼吸道冠状病毒病毒猪流感病毒 A 型病毒猪呼吸系统疾病 猪呼吸系统疾病

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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