在脂多糖 (LPS) 刺激下,巨中circDNAJB6的表达,功能和网络调节
Jishuang Tan1, Paizelaiti Sahaer1, Huan Li2
1College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Developmental and comparative immunology
|November 11, 2023
概括
圆形RNA DNAJB6 (circDNAJB6) 通过影响细胞活力和炎症反应,加剧了细菌感染引起的损伤. 这项研究强调了circDNAJB6作为潜在的生物标志物和细菌感染的治疗点.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 循环RNAs (circRNAs) 是基因表达的关键调节者,影响转录,拼接和翻译.
- 肉circRNAs在病变发生中的作用在很大程度上仍未被探索.
- 圆形RNA DNAJB6 (circDNAJB6) 之前在细菌感染期间在巨细胞中被发现.
研究的目的:
- 研究巨中circDNAJB6的功能和分子机制.
- 确定circDNAJB6在细菌感染引起的细胞损伤中的作用.
- 通过circDNAJB6.6调节的潜在目标和路径的识别.
主要方法:
- 使用PCR放大,桑格测序和RNase R处理来识别和描述circDNAJB6.
- 对circDNAJB6对脂聚糖 (LPS) 诱导的细胞损伤的影响的评估.
- 生物信息分析用于预测miRNA相互作用和目标基因.
主要成果:
- circDNAJB6通过降低细胞活力和增加氧化和促炎性细胞因子的产生,加剧LPS诱导的细胞损伤.
- 生物信息分析揭示了circDNAJB6和五个miRNA之间的相互作用,针对75个基因.
- 丰富的途径包括自和MAPK信号传递,这表明它们在细胞压力和炎症中的作用.
结论:
- circDNAJB6在加剧细菌感染引起的细胞损伤方面发挥着重要作用.
- circDNAJB6可能通过调节miRNA路径和影响自和MAPK信号来发挥作用.
- circDNAJB6为治疗家禽细菌感染提供了潜在的生物标志物和治疗点.
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