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单细胞ICP-MS用于评估Cd,Ce和U与Streptomyces coelicolor子的相互作用行为

Steffen Hellmann¹, Paula García-Cancela², Sergio Alonso-Fernández³

¹Institute of Geosciences, Friedrich Schiller University Jena, Burgweg 11, 07749, Jena, Germany; International Max Planck Research School for Global Biogeochemical Cycles, Max Planck Institute for Biogeochemistry, Department of Biogeochemical Processes, Hans-Knöll-Straße 10, 07745, Jena, Germany.

|November 11, 2023

概括

菌细菌通过吸附和封装与 (Ce) 和 (U) 等金属相互作用,但不与 (Cd) 相互作用. 这种金属选择性是用于污染土壤生物修复中使用Streptomyces的关键.

科学领域:

环境微生物学环境微生物学
生物修复是一种生物修复.
细菌生理学细菌生理学

背景情况:

杆菌是土壤细菌,对于金属污染场所的生物修复至关重要.
了解Streptomyces中的金属选择性和相互作用机制对于优化它们的应用至关重要.
目前关于Streptomyces如何与特定金属相互作用的知识是有限的.

研究的目的:

为了研究Streptomyces coelicolor子的金属选择性吸收和吸附机制.
为了确定Ce,U和Cd与Streptomyces子和的相互作用.
阐明吸附与代谢吸收在金属协会中的作用.

主要方法:

暴露S. coelicolor子在固体和水性介质中的不同度的Ce,U和Cd.
使用子清洗处理 (Tris缓冲器与Tris-EDTA缓冲器) 区分弱吸收和强吸收金属.
使用单细胞 (sc) ICP-MS.量化单个子中的金属含量.

主要成果:

观察到金属对不活性子的元素特异性吸附,Ce和U的吸附显著强,Cd的吸附中等.
在金属改性固体介质中,Ce和U与子强烈相关,而Cd低于检测极限.
发芽的叶有效地封装了Ce和U,但没有Cd,这表明了差异化的代谢相互作用.

结论:

甲状腺菌的子金属相互作用始于吸附,Ce,U和Cd显示不同程度的关联.
发芽的Streptomyces hyphae表现出选择性的金属封装,有效地与Ce和U相互作用,但不包括Cd.
这项研究提供了对金属与Streptomyces特异性相互作用的关键见解,增强了它们在生物修复中的有针对性的使用.

关键词:

协会协会协会协会协会生物积累生物积累生物积累是的组成部分.(Cerium) 是一种有机物.格拉姆阳性细菌是什么?是一种.

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