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与天然芳香性化物交叉连接果衍生DNA,产生膜解抗菌纳米凝

Fang-Yu Chung¹, Yi-Zhen Lin¹, Cheng-Rung Huang²

¹Master Program in Biomedicine, National Taitung University, No. 684, Section 1, Zhonghua Rd., Taitung 95092, Taiwan.

International journal of biological macromolecules

|November 11, 2023

概括

研究人员从果DNA和天然化物开发了新的纳米凝. 这些生物相容的纳米凝对耐药细菌表现出强大的抗菌活性,为新型抗生素提供了一个有希望的绿色替代品.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
抗菌研究抗菌研究

背景情况:

抗生素耐药细菌的增加,特别是在生物膜中,需要开发新的抗菌战略.
寻求具有高生物相容性和低毒性的绿色材料作为传统抗生素的替代品.

研究的目的:

用基维果衍生DNA和芳香性化物合成和表征新型纳米凝 (NGs).
评估这些DNA芳香性化物NGs的生物相容性和抗菌功效.

主要方法:

纳米凝通过水在素乳液合成,将DNA的氨基与芳香性化物 (甲,p-anisaldehyde, vaniillin) 交叉连接.
使用传输电子显微镜和泽塔电位测量进行了表征.
对正常细胞有机体和人类红细胞 (红细胞) 的细胞毒性测定.
对四种致病细菌进行抗菌检测,包括最低抑制度 (MIC) 的确定.
扫描电子显微镜 (SEM) 和免疫阻塞用于分析细菌损伤和蛋白质表达.
在体内使用*C. elegans*模型进行有效性测试.

主要成果:

合成的球形NGs (40-140nm) 带有负泽塔潜力.
证明对正常细胞和红细胞的低细胞毒性.
DNA-瓦尼林 (DNA-VA) NGs显示出显著的抗菌活性,MIC值较低.
SEM揭示了细菌变形,免疫染显示了改变的groEL蛋白表达.
*C. elegans*的生存率在抗击P. aeruginosa*感染时得到了提高.

结论:

DNA芳香性化物NGs具有生物相容性,并表现出强大的广泛抗菌特性.
纳米凝结构增强了绑定的抗菌化物的有效性.
这些发现为开发绿色,有效的抗微生物药物来对抗耐药细菌提供了一个新的平台.

关键词:

抗菌纳米凝是一种抗菌的纳米凝.芳香性类型的阿尔代化物.抗药性菌株是一种抗药性菌株.绿色材料绿色材料

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