Marsupials 具有单基MEST表达与保存的反意义 lncRNA,但MEST没有印记
Teruhito Ishihara1,2, Shunsuke Suzuki3, Trent A Newman1
1School of BioSciences, The University of Melbourne, Melbourne, VIC, 3010, Australia.
Heredity
|November 11, 2023
概括
这就是MEST基因.
科学领域:
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 进化生物学 进化生物学
背景情况:
- MEST基因的印记短异型影响哺乳动物的发育.
- 类哺乳动物在MEST促进体上表现出差异甲基化区域 (DMR),与有袋动物不同.
- 了解MEST印记进化需要对不同哺乳动物群体进行比较基因组分析.
研究的目的:
- 为了研究MEST基因在哺乳动物之间印记的进化起源.
- 为了比较MEST基因位点在一个有袋动物 (tammar wallaby),单体动物 (白) 和eutherian (老鼠) 中.
- 确定保存的监管元素在MEST印记中的作用.
主要方法:
- 在选择的哺乳动物物种中对MEST基因位点进行比较分析.
- 证实了短MEST异型在有袋动物,单动物和eutherians的存在.
- 检查促进体区域,CpG岛屿和相关的非编码RNA.
主要成果:
- 在所有研究的哺乳动物群体中都保留了短的MEST异型.
- 一个保存的反意义 lncRNA 和 CEP41 异型共享一个假定的促进者 CpG 岛与 Tammar wallabies 中的短 MEST 异型.
- 与之前的假设相反,在有袋动物中没有检测到MEST基因印记.
结论:
- 短的MEST异型的一个替代促进体可能在哺乳动物的共同祖先中进化.
- 在袋鼠-袋鼠分歧后,MEST印记在eutherians中出现,涉及获得DMR.
- 保存的 lncRNA 可能在建立 MEST 印记的过程中发挥作用,但在有袋动物中没有印记.
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