通过药物诱导的ROS通过细胞骨脱聚合的纳米孔相关的细胞死亡
Yan Zhang1, Renfeng Xu1, Jingjing Wu2
1Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China.
Talanta
|November 12, 2023
概括
原子力显微镜可视化了前列腺癌细胞中帕克利塔塞尔诱导的毛孔. 反应性氧物种驱动孔隙形成,为评估癌症药物治疗提供了一种新方法.
科学领域:
- 在瘤学瘤学.
- 纳米技术纳米技术
- 细胞生物学 细胞生物学
背景情况:
- 前列腺癌 (PCa) 是一种普遍存在的恶性瘤,目前正在研究药物疗效和机制.
- 目前的治疗策略需要进一步探索分子层面的药物细胞相互作用.
研究的目的:
- 通过原子力显微镜 (AFM) 对前列腺癌细胞研究帕克利塔塞尔 (PTX) 的抗癌机制.
- 探索反应性氧物种 (ROS) 在PTX诱导的细胞损伤和膜变化的作用.
主要方法:
- 使用AFM可视化PTX处理后PC3M细胞膜的形态变化.
- 量化孔隙形成的直径,深度和数量,以一种依赖于度和时间的方式.
- 通过使用ROS清理器并评估其对孔隙形成的影响来研究ROS的参与.
主要成果:
- 在帕克利塔塞尔治疗后,AFM揭示了PC3M细胞膜上的孔状结构的形成.
- 孔隙形成与PTX暴露具有度和时间依赖的关系.
- 反应性氧气物种被确定为在脱聚化actin细胞骨的关键媒介,导致膜损伤和孔隙诱导.
- 使用ROS清除剂进行预处理有效抑制了帕克利塔塞尔诱导的孔形成.
结论:
- AFM提供了一种新的纳米级成像方法,用于评估药物对癌细胞的影响.
- 帕克利塔塞尔通过ROS介导的机制诱导细胞膜损伤,涉及actin细胞骨脱聚合.
- 这项研究为前列腺癌的药物向治疗评估提供了新的视角.
相关概念视频
01:16Necrosis
4.6K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
4.6K
01:30Overview of Cell Death
7.3K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
7.3K
01:18Autophagic Cell Death
3.4K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.4K