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从冠状病毒1a/1b蛋白基因衍生出的亚基因mRNA的鉴定:对冠状病毒转录的含义

Ching-Hung Lin¹, Feng-Cheng Hsieh¹, Meilin Wang²

¹Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, Taichung, 40227, Taiwan.

|November 12, 2023

概括

冠状病毒亚基因组mRNA (sgmRNA) 合成需要在转录调节序列 (TRS-B) 中从病毒基因组中分离聚合酶. 在聚合酶解离后,领导TRS (TRS-L) 和TRS-B之间的序列同质性对于高效的1ab/sgmRNA生产至关重要.

科学领域:

病毒学病毒学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

冠状病毒亚基因组mRNA (sgmRNA) 合成对于病毒复制至关重要,并由转录调节序列 (TRS) 调节.
1ab/sgmRNA是由1a/1b蛋白基因中的身体TRS (TRS-B) 合成的,但控制其产生的精确机制仍然不完全理解.

研究的目的:

为了全面识别来自TRS-Bs合成的1ab/sgmRNAs,跨越不同冠状病毒基因.
调查影响1ab/sgmRNA合成的因素,特别是领导TRS (TRS-L) 和TRS-B之间的序列同质性的作用.
改进对冠状病毒转录机制的理解.

主要方法:

逆转录聚合酶链反应 (RT-PCR) 和测序被用来识别和表征1ab/sgmRNAs.
在体外和体外实验系统被用来研究各种冠状病毒的sgmRNA合成.

主要成果:

该研究确定了不同冠状病毒基因中的TRS-Bs合成的1ab/sgmRNAs.
结果表明,TRS-L和TRS-B之间的序列同质性可能不是1ab/sgmRNA合成的主要决定因素.
提出了新型冠状病毒转录模型,强调了聚合酶解离的重要性.

结论:

冠状病毒聚合酶与TRS-B中的病毒基因组分离是sgmRNA合成的先决条件.
在聚合酶解离之后,TRS-L和TRS-B之间的序列同质性对于高效的sgmRNA合成至关重要.
这项研究促进了对冠状病毒转录调节和sgmRNA生物发生的理解.

关键词:

新冠病毒新冠病毒新冠病毒.冠状病毒基因组结构冠状病毒转录的转录基因表达基因表达基因表达在亚基因组mRNA中.转录调节序列的编写.

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