经济和环境影响评估的早期阶段,以优化生物工艺的发展:单类醇类 monoterpenoid
Deniz Etit1, Ólafur Ögmundarson2, Jie Zhang1
1Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs. Lyngby, Denmark.
Bioresource technology
|November 12, 2023
概括
微生物重构可以可持续地生产来自植物的药品. 这项研究模拟了酵母中的tabersonine生产,优化了流程以降低成本和环境影响.
科学领域:
- 生物技术和代谢工程 生物技术和代谢工程
- 化学工程是化学工程的重要组成部分.
- 环境科学 环境科学
背景情况:
- 植物制药面临着高昂的生产成本,环境破坏和供应链问题.
- 微生物重构为制药生产提供了一个可持续的替代方案.
- 早期阶段 (TRL 3-5) 的经济和环境评估对于生物过程优化至关重要.
研究的目的:
- 为了模拟Saccharomyces cerevisiae中的微生物tabersonine生产.
- 引导生物制药的工艺规模经济和环境优化.
- 进行综合技术经济分析 (TEA) 和生命周期评估 (LCA).
主要方法:
- 在早期发育阶段 (TRL 3-5),对Saccharomyces cerevisiae中的微生物tabersonine生产进行建模.
- 技术经济分析 (TEA) 用于确定最低售价 (MSP).
- 生命周期评估 (LCA) 用于量化全球变暖潜力 (GWP).
主要成果:
- 在0.7mg/L的基准情况生产中,MSP为3,910,000美元/公斤,GWP为2,540公斤CO2eq/g.
- 工业尺度 (1g/L) 将MSP降至4,262美元/公斤,GWP降至6.36公斤CO2eq/g.
- 连续加工和现场产品提取大大降低了MSP和GWP.
结论:
- 早期的TEA-LCA建模对于优化生物制药生产至关重要.
- 微生物重构为制药制造提供了一个可持续和经济可行的途径.
- 过程改进,如连续加工和现场提取,是提高可持续性和降低成本的关键.
相关概念视频
Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
Production of Antibiotics
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
Production of Pharmaceuticals
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...
Biofuels
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...


