对四种大西洋鱼 (Salmo salar) serpinh1的特征和转录表达分析提供了进化分歧的证据
Eric H Ignatz1, Jennifer R Hall2, Khalil Eslamloo1
1Department of Ocean Sciences, Memorial University of Newfoundland and Labrador, 0 Marine Lab Road, St. John's, NL A1C 5S7, Canada.
Gene
|November 12, 2023
概括
这项研究描述了大西洋鱼中的四种serpinh1基因对应物,揭示了不同的表达模式和对环境压力因素的反应. 这些发现支持serpinh1b-1和serpinh1b-2作为鱼热应激生物标志物.
科学领域:
- 基因组学和分子生物学
- 水产养殖和鱼类生理学
- 进化生物学 进化生物学
背景情况:
- 大西洋鱼 (Salmo salar) 是经济上至关重要的养殖鱼,也是研究全基因复制 (WGD) 后的基因进化有价值的模型.
- 分子陪伴者serpinh1对原蛋白完整性至关重要,也是鱼类中已知的热应激生物标志物.
研究的目的:
- 在核酸和氨基酸水平上描述四种大西洋鱼的类型.
- 在各种组织和条件下调查这些类比的构成性和压力诱导的表达模式.
主要方法:
- 克隆和测序四个大西洋三文鱼的serpinh1类似物.
- 在核酸和氨基酸序列的分析.
- 定量PCR (qPCR) 用于评估17种组织的基因表达,以应对压力表型,温度变化 (12°C对20°C) 和疫苗接种.
主要成果:
- 在17个组织中,serpinh1a-2表现出独特的构成性表达特征.
- 疫苗注射显著上调了大多数serpinh1对应物 (1.1至4.5倍).
- 与12°C相比,高温 (20°C) 显示了serpinh1a-1/a-2的表达较低,而serpinh1b-1/b-2的表达显著增加 (10.2至19.0倍).
结论:
- 支持serpinh1b-1和serpinh1b-2作为大西洋鱼热应激的潜在生物标志物.
- 有证据表明,serpinh1类似物之间存在新功能和/或子功能,这为WGD后的进化分歧提供了洞察力.
- 这项研究为了解鱼类热耐受机制中serpinh1的作用提供了基础.
相关概念视频
Multi-species Conserved Sequences
3.9K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
3.9K
Convergent Evolution
27.8K
Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure leads to the rise of similar but unrelated adaptations in organisms with no recent common ancestors, a process known as convergent evolution.
27.8K
Evolutionary Relationships through Genome Comparisons
5.8K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.8K
Cis-regulatory Sequences
9.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.9K
Gene Duplication and Divergence
6.1K
The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
6.1K
Gene Evolution - Fast or Slow?
7.1K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
7.1K


