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来自Salmo salar的TLR5中截断的HMGB1酸尾序列的酸的对抗作用
Aleikar Vásquez-Suárez1, Leonardo Ortega1, Iván González-Chavarría1
1Biotechnology and Biopharmaceuticals Laboratory, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Víctor Lamas 1290, Concepción, CP. 4030000, Chile.
Fish & shellfish immunology
|November 12, 2023
概括
来自Salmo salar HMGB1的新型体显示出作为TLR5激动剂的潜力. 5LWK有效地刺激免疫反应,包括细胞因子的产生,这表明它可以作为鱼类的免疫刺激剂.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 水产养殖是水产养殖的一种方式.
背景情况:
- 收费类受体5 (TLR5) 通过识别细菌鞭毛体,在天生的免疫力中起着至关重要的作用.
- 开发新型免疫刺激剂对于增强水产养殖中的鱼类健康和抗病能力至关重要.
- 高流动性组盒子1 (HMGB1) 蛋白质碎片已经显示出免疫调节活性.
研究的目的:
- 设计和评估从萨尔莫沙拉 HMGB1.1. 的酸性尾巴中提取的新型TLR5激动性.
- 评估这些作为S. salar. 免疫刺激剂的潜力.
主要方法:
- 使用对TLR5ectodomain的盲目对接平台进行in silico酸设计.
- 精选的化学合成 (6WK,5LWK,SsOri) 和功能测试.
- 在鱼细胞中评估NF-κB通路激活 (p-NF-κBp65,核转位) 和促炎性细胞因子表达 (TNFα,IL-1β,IL-8).
主要成果:
- 6WK和5LWK被确定为潜在的TLR5激动剂.
- 5LWK显著诱导NF-κBp65的核转位,并在S. salar头细胞 (HKLs) 中调高了亲炎性细胞因子转录.
- 虽然这两种都激活了NF-κB信号,但与6WK和SsOri相比,5LWK对免疫刺激的影响更为明显.
结论:
- 来自S. salar HMGB1酸尾的新可以作为TLR5激动剂.
- 5LWK显示出作为一种有效的免疫刺激剂的显著潜力,用于增强Salmo salar的先天免疫力.
- 这些发现支持开发用于水产养殖的基于的免疫疗法.
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