通过使用iPBS-SCoT标记方法对原生花遗传资源的遗传多样性进行评估,并通过使用DNA条形码方法对物种进行鉴定
Halil Ibrahim Sagbas1, Sezai Ercisli2, Murat Aydin3
1Faculty of Agriculture, Department of Horticulture, Ataturk University, TR-25240, Erzurum, Türkiye. hibrahimsagbas@gmail.com.
Cellular and molecular biology (Noisy-le-Grand, France)
|November 13, 2023
概括
这项研究使用Inter Primer Binding Site (iPBS) 和Start Codon Target Polymorphism (SCoT) 标志物揭示了黑种群中显著的遗传多样性. DNA 条形码确认了不同的基因型,强调了保护这些宝贵的药用植物资源的必要性.
科学领域:
- 植物遗传学 植物遗传学
- 药用植物学 药用植物学
- 分子生态学分子生态学
背景情况:
- 棘 (Crataegus spp.) 是一种棘. 是一种全球公认的药用植物,在传统中医中具有丰富的历史.
- 它的各个部分,包括花朵,叶子和水果,因其丰富的植物化学成分和健康益处而受到重视.
- 了解棘的遗传多样性对于其可持续利用和保护至关重要.
研究的目的:
- 用分子标记物评估来自土耳其的101种棘基因型中的遗传变异.
- 执行DNA条形码,用于准确的物种识别和遗传分析.
- 强调保护药用植物遗传资源的重要性.
主要方法:
- 使用Inter Primer Binding Site (iPBS) 和Start Codon Target Polymorphism (SCoT) 标记器进行遗传多样性分析.
- 用ITS标记器进行基因识别的DNA条形码.
- BLASTN对NCBI数据库进行BLASTN搜索,以进行基因型比较和相似性评估.
主要成果:
- iPBS标记物在10个原始体中确定了400个等位基因,多态信息含量 (PIC) 值从0.239到0.272.
- 在SCoT原料中,平均发现了50.05个等位基因,PIC值在0.251到0.297.7之间.
- DNA条形码证实了基因型的准确性,NCBI对14个不同的加入的相似率在90.83%至100%之间.
结论:
- 在棘基因型中存在显著的遗传多样性,特别是在种子传播的种群中.
- 像iPBS和SCoT这样的分子标记物是评估黑的遗传变异的有效工具.
- 保护工作对于保护这一重要的药用植物的遗传资源至关重要.
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