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生物合成的银纳米粒子通过抑制NF-κB信号通路来抑制骨质细胞形成

Yu Wu^1,2,3, Zhong Cheng^2,3, Wenhui Hu¹

¹Department of Biomedical Materials Science, Third Military Medical University, Chongqing, 400038, China.

Advanced biology

|November 13, 2023

概括

生物合成的银纳米粒子 (Ag NPs) 通过清除活性氧物种 (ROS) 有效地治疗炎症性骨损失. 这些AgNP降低骨质细胞活性,改善骨密度,提供了一个有前途的治疗方法.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
免疫学免疫学免疫学

背景情况:

过度活跃的骨质细胞驱动炎症性骨损失.
反应性氧物种 (ROS) 加剧骨质细胞的形成和功能.
银纳米粒子 (Ag NPs) 显示出因ROS清理性质而减轻骨损失的潜力.

研究的目的:

使用Flos Sophorae Immaturus提取物合成和表征低毒性,稳定的AgNP.
在炎症性骨损失的体外和体外模型中评估这些AgNP的疗效.
调查AgNP对骨质细胞形成和骨密度的作用的潜在机制.

主要方法:

使用Flos Sophorae Immaturus提取物进行AgNP的生物合成.
建立一个LPS诱导的炎症性骨损失模型.
定量逆转录-聚合酶连锁反应 (RT-qPCR) 和西蒙测试用于评估基因和蛋白质表达.
在体内对耐酸酸酶阳性 (TRAP+) 细胞和骨密度的评估.

主要成果:

合成的AgNP显示出低毒性和高稳定性.
AgNP显著降低了TRAP+细胞的数量,并降低了关键骨质结晶生物标志物的下调.
通过抑制NF-κB通路的ROS介导酸化,AgNP抑制了骨质细胞形成.
在体内研究表明,Ag NPs改善了骨密度和骨质细胞数量的减少.

结论:

生物合成的AgNP有效地抑制骨质细胞的形成和活动.
通过降低ROS和NF-κB信号的调节,AgNP可以减轻炎症性骨损失.
这些发现强调了AgNP作为炎症性骨损失的潜在治疗剂.

关键词:

这是一种Flos Sophorae Immaturus提取物.炎症性骨损失导致的骨损失骨质结晶体是骨质结晶体兰克尔 (Rankl) 是一个名字.银纳米颗粒银纳米粒子

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