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延迟发芽1 (DOG1) 样蛋白调节的子发芽 在的Physcomitrium patens
Evelyn Vollmeister1, Alexandros Phokas2, Rabea Meyberg1
1Plant Cell Biology, Department of Biology, University of Marburg, Marburg, Germany.
The Plant journal : for cell and molecular biology
|November 13, 2023
概括
发芽延迟1 (DOG1) 蛋白质在调节种子休眠中的功能在子芽中得到保护. PpDOG1-LIKE1与DELLA蛋白相互作用,这表明植物谱系中发芽控制的共享机制.
科学领域:
- 植物生物学 植物生物学
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
背景情况:
- 发芽延迟1 (DOG1) 对于开花植物的种子休眠和发芽至关重要.
- 类植物,像一样,利用具有类似于种子的功能的平分体子.
- 了解跨植物群的DOG1保护,可以阐明基本的发芽机制.
研究的目的:
- 为了研究DOG1在植物子发芽中的保存功能.
- 为了分析DOG1在的作用的分子机制,Physcomitrium patens.
- 为了探索的DOG1同类与DELLA蛋白的相互作用.
主要方法:
- 遗传学和in silico表达分析以确定P. patens.中的DOG1域基因.
- 在Ppdog1-like1突变和与Arabidopsis thaliana DOG1 (AtDOG1) 补充的发芽测试.
- 酵母双杂交试验,以评估PpDOG1-L1和DELLA蛋白之间的蛋白质-蛋白质相互作用.
主要成果:
- 菲斯科米特里姆病原体有9个DOG1域含有的基因.
- 类DOG1类蛋白质PpDOG1-L1与类DELLA蛋白质 (PpDELLAa,PpDELLAb) 和类DELLA蛋白质 (AtRGA) 相互作用.
- DOG1 域对于与 DELLA 蛋白相互作用至关重要且足够;Ppdog1-l1 突变体子显示加速发芽,表型因 AtDOG1 表达而逆转.
结论:
- PpDOG1-LIKE1在子子发芽中发挥作用,可能与PpDELLAs相互作用.
- DOG1 域在不同植物群体的发芽调节方面表现出保留的功能.
- 监管网络的差异性适应是种子与子发芽控制的基础.
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