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单细胞测序和转录组分析用于构建一种与流体相分离相关的风湿性关节炎基因模型

Jiaojiao Tai¹, Linbang Wang², Ziqiang Yan¹

¹Department of Orthopedics, Honghui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Frontiers in genetics

|November 13, 2023

概括

液-液相分离 (LLPS) 失调可能导致类风湿性关节炎 (RA). 研究人员确定MYC和MAP1LC3B是关键基因,可能成为这种自身免疫性疾病的新生物标志物.

科学领域:

生物化学生化学
免疫学免疫学免疫学
遗传学是一个遗传学.

背景情况:

类风湿性关节炎 (RA) 是一种使人虚弱的自身免疫性关节疾病.
液-液相分离 (LLPS) 的失衡与炎症和癌症有关.
对于LLPS在RA发病过程中的具体作用尚不清楚.

研究的目的:

研究LLPS相关基因在类风湿性关节炎中的作用.
用生物信息学和分子技术识别RA的潜在分子标记物.

主要方法:

从RA和正常样本中分析转录组和单细胞测序数据.
应用机器学习算法和加权基因共同表达网络分析来识别枢纽基因.
使用qRT-PCR验证枢纽基因表达和与免疫细胞透的相关性分析.

主要成果:

36个与LLPS相关的基因在RA中被差异地表达.
MYC和MAP1LC3B被确定为具有高诊断精度 (AUC高达0.911) 的关键枢纽基因.
在RA突组织中,MYC和MAP1LC3B的表达显著较低,与免疫细胞透相关.

结论:

在类风湿性关节炎的发病过程中,LLPS可能发挥重要作用.
MYC和MAP1LC3B是潜在的RA新型分子标记物.
这些基因具有免疫学意义,可以为未来的治疗策略提供信息.

关键词:

在MAP1LC3B中,我们可以看到我的世界 MYC 生物信息学是一种生物信息学.液态-液态相隔离方法液态相隔离方法类风湿性关节炎类风湿性关节炎一个单细胞测序.转录组 (transcriptome) 是一个转录组.

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