Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Atypical Hemolytic Uremic Syndrome in Children and Adults With the Hot Spot C3 Gene Variant p.Arg161Trp.

Kidney international reports·2026
Same author

Unveiling Donor-Derived BKPyV DNAemia Through Analysis of Contralateral Kidney Transplant Recipients.

Biomedicines·2026
Same author

Kidney Transplant Outcomes in Patients with and without Type II Diabetes Mellitus.

Cardiorenal medicine·2026
Same author

Editorial: Newborn screening for inborn errors of metabolism volume II.

Frontiers in pediatrics·2026
Same author

Novel Allocation Strategies Can Boost Kidney Exchange Programs: A Monte Carlo Simulation.

Transplant international : official journal of the European Society for Organ Transplantation·2026
Same author

Interaction between HLA-B leader peptide variants and cytomegalovirus serostatus is associated with early T cell-mediated rejection in kidney transplantation.

Frontiers in immunology·2026

相关实验视频

Updated: Jul 11, 2025

Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation
06:39

Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation

Published on: August 19, 2020

5.7K

模型对人类球微血管内皮细胞的补充激活.

Kes H Stevens1, Laura M Baas1, Thea J A M van der Velden1

  • 1Department of Pediatric Nephrology, Amalia Children's Hospital, Radboud University Medical Center, Nijmegen, Netherlands.

Frontiers in immunology
|November 13, 2023
PubMed
概括

一个新的ex vivo模型有效地测量了非典型血清性尿素综合征 (aHUS) 中的补充沉积. 这种工具有助于理解HUS的病理生理学和脏疾病中的内皮补充激活.

关键词:
在C5b-9中使用.另一个替代路径路径.非典型的血液溶解尿素性尿性综合征.互补系统是互补的系统.在此之前,Eculizumab也曾被使用过.质内皮质的质内皮质

更多相关视频

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells
10:23

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells

Published on: November 4, 2022

3.1K
Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

17.7K

相关实验视频

Last Updated: Jul 11, 2025

Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation
06:39

Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation

Published on: August 19, 2020

5.7K
Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells
10:23

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells

Published on: November 4, 2022

3.1K
Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

17.7K

科学领域:

  • 腎臟病學 (nephrology) 是一種醫學專業.
  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学

背景情况:

  • 非典型的血溶性尿素性综合征 (aHUS) 是一种罕见的病.
  • 它源于补充替代途径的失调,损害了质内皮.
  • 了解aHUS的病理生理学需要对内皮补充体沉积的有效模型.

研究的目的:

  • 为了验证测量补体沉积的ex vivo模型.
  • 从对照和aHUS患者对人类质微血管内皮细胞 (GMVECs) 进行补充沉积的评估.
  • 调查这个模型在研究 aHUS.US 中的实用性.

主要方法:

  • 人类测试血清被用GMVECs化.
  • 使用免疫光显微镜可视化和量化补充沉积 (C5b-9).
  • 该模型使用齐莫桑激活血清,NHS和C5抑制剂eculizumab进行了验证.

主要成果:

  • 该模型显示,与NHS相比,齐莫桑激活血清中的C5b-9沉积增加.
  • 非典型的血溶性尿素性综合征患者的血清在急性阶段表现出C5b-9沉积的升高.
  • 缓解样本显示正常化沉积,模型对eculizumab治疗有反应.

结论:

  • 开发了一个强大的外体模型来测量C5b-9补充沉积在GMVEC上.
  • 这个模型准确地反映了aHUS中的补体沉积.
  • 该模型是研究aHUS和其他涉及内皮补充激活的疾病的宝贵工具.