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Updated: Jul 11, 2025

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
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Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine

Published on: February 16, 2018

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酒精脱水酶的酶选择性源的起源

Jiahui Zhou1, Tao Han2, Shahbaz Ahmad1,3,4

  • 1School of Chemistry and Chemical Engineering, Queen's University, David Keir Building, Stranmillis Road, Belfast BT9 5AG, Northern Ireland, UK. m.huang@qub.ac.uk.

Physical chemistry chemical physics : PCCP
|November 13, 2023
PubMed
概括

研究人员设计了酒精脱酶 (ADHs) 来脱对称环-1,3-,产生特定的立体异构体. 分子动力学和QM/MM揭示了酶机制和酶选择性的起源,用于合理的酶工程.

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As depicted in the figure below, the unsymmetrical ketones can form two possible enolates:  less substituted or more substituted enolates. Usually, the thermodynamic enolates are formed from the more substituted α-carbon atom, while the kinetic enolates are formed faster by deprotonation from the less substituted position. The thermodynamic enolates have lower energy, so they are  more stable. But the energy required to form kinetic enolates is less.
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