人体肠道树突细胞可以克服视网膜酸信号,产生具有肠道和皮肤均具有宿命性质的 CD4 T 细胞
Hannah Gordon1, Katherine Wichmann1, Amy Lewis2
1Centre for Immunobiology, Blizard Institute, Faculty of Medicine and dentistry, Barts and The London Medical School, Queen Mary University of London, London, United Kingdom.
Journal of immunology (Baltimore, Md. : 1950)
|November 13, 2023
概括
肠道树突细胞 (DCs) 产生双热带T细胞,这些T细胞流向肠道和皮肤. 这些表达alpha4beta7和皮肤白细胞抗原 (CLA) 的细胞在克罗恩病中增加,可能会在两个位置引起炎症.
科学领域:
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
- 皮肤病学 皮肤病学
背景情况:
- 来自肠道树突细胞 (DCs) 的网红酸通过α4β7整合素将T细胞引导到肠道,抑制皮肤的回归.
- 传统的T细胞激活通常会导致肠道或皮肤热带,但不是两者兼而有之.
研究的目的:
- 研究具有双肠和皮肤贩运潜力的T细胞的生成和特征.
- 确定肠道DC和特定分子途径在产生这些双热带细胞中的作用.
- 探索双热带T细胞在炎症性肠病中的存在和意义.
主要方法:
- 在有或没有肠道DC的情况下,人类原始CD4T细胞的激活.
- 流细胞计用于分析T细胞表面标记物表达 (alpha4beta7,CLA,alpha4beta1).
- 对于FUT7表达的定量实时PCR.
- 从克罗恩病患者的肠道组织单细胞RNA测序.
主要成果:
- 肠道DC激活产生CLA+alpha4beta7+"双热带"T细胞,也表达alpha4beta1整体.
- 需要细胞接触并由IL-12/IL-23增强的FUT7上调,对于DC诱导的CLA+alpha4beta7+T细胞生成至关重要.
- 血液中CD4+ T细胞包括用于细胞因子生产的CLA+alpha4beta7+细胞 (IFN-,IL-17,TNF-alpha).
- 双热带淋巴细胞在克罗恩病肠道组织中增加,在炎症区域有明显的FUT7表达,增殖细胞群.
结论:
- 肠道DCs诱导了一种独特的双热带T细胞群,具有潜力透肠道和皮肤.
- 这些双热带T细胞与炎症性肠病的发病有关,可能导致肠道和皮肤表现.
- 这些细胞具有FUT7表达和特定细胞因子的特征,突出显示它们在免疫反应和炎症中的作用.
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