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一种基于类似欧米茄的DNA纳米结构的均无标签的电化学感应传感器,用于检测孕激素

Zaofen Wang¹, Weiping Shi¹, Yunzhu Tan¹

¹Guizhou Engineering Laboratory for Synthetic Drugs (Ministry of Education of Guizhou Province), College of Pharmacy, Guizhou University, Guiyang 550025, China. nqliu@gzu.edu.cn.

Analytical methods : advancing methods and applications

|November 13, 2023

概括

这项研究介绍了一种用于敏感的孕激素检测的新型电化学感应传感器. 该传感器利用类似欧米茄的DNA纳米结构和外核酶III放大,实现了低检测极限.

科学领域:

生物传感器和纳米技术
电化学分析电化学分析
分子诊断学分子诊断学

背景情况:

孕激素检测在各种生物和临床应用中至关重要.
现有的孕激素检测方法往往面临敏感性,特异性或复杂性的局限性.
开发快速,灵敏和无标签的检测平台是非常理想的.

研究的目的:

开发一种新的同质无标签的电化学感应传感器,用于敏感的孕激素检测.
为了整合一个类似欧米茄的DNA纳米结构与同热循环放大策略.
为了利用外核酶III (ExoIII) 增强信号放大和aptamer回收利用.

主要方法:

制造一种类似欧米茄的DNA (Ω-DNA) 纳米结构,以使孕激素体不动化.
实施使用EXO III进行信号放大的异热循环放大策略.
通过基于DNA酶活性的微分脉冲电量计 (DPV) 检测孕激素.

主要成果:

胃感应器显示出对孕激素的敏感反应,具有广泛的动态线性范围,从1 pg mL−1到10 ng mL−1.1.
在最佳条件下,可以达到0.3 pg mL-1的低检测极限.
试验显示,由于DNA酶形成减少,孕激素存在的信号减少.

结论:

开发的电化学适应传感器为孕激素检测提供了一个灵敏而可靠的平台.
Ω-DNA纳米结构和Exo III放大组合为生物传感提供了一个有效的策略.
这种方法有可能在临床诊断和生化分析中得到实际应用.

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