拼接因子Prp18p促进共识3'-拼接位点的全基因组忠实性
Kevin R Roy1,2, Jason Gabunilas1, Dean Neutel1
1Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.
Nucleic acids research
|November 13, 2023
概括
拼接因子 Prp18p 确保准确的 3'拼接位点 (3'SS) 识别. 没有Prp18p,酵母结合体错误地使用替代的,非正规的3'SS序列,影响基因表达.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 生物化学 生物化学
背景情况:
- 精确的拼接部位选择对于基因表达至关重要.
- 结合体对低复杂性3共识序列 (YAG) 的识别是一个挑战.
- 拼接因子 Prp18p 在保持拼接部位忠实性方面发挥着作用.
研究的目的:
- 为了研究 Prp18p 在 3 个缩写{SS} 忠诚度中的作用.
- 为了识别 Prp18p.p. 缺席的其他 3 个缩写{SS} 的使用情况.
- 阐明 Prp18p 介导的拼接部位选择背后的分子机制.
主要方法:
- 在Saccharomyces cerevisiae (S. cerevisiae) 中进行遗传分析.
- 对替代3个缩写{SS}激活的分析.
- 研究拼接因子 (Prp18p,Slu7p,Prp8p,Prp22p,Prp43p) 之间的相互作用.
主要成果:
- 缺少Prp18p会导致全基因组激活替代3个缩写{SS},包括非YAG序列.
- 非正规的3个缩写{SS}的使用是由上游的多个 (U) 段和与第一个内基核oside的相互作用增强的.
- Prp18p的忠实功能涉及与Slu7p和Prp8p的相互作用,并通过Prp22p酶活性进行协同作用.
结论:
- 酵母结合酶体表现出放松的3序列使用,没有Prp18p.
- 以Prp18p为中心的结合体相互作用网络对于识别共识3序列至关重要.
- 在拼接过程中,Prp18p在确保3个缩写{SS}的忠实性方面发挥着独特而至关重要的作用.
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