强化和空间可控制的神经元活动,由跨的聚焦超声波刺激诱导,与相变纳米滴相结合
Mengke Wang1, Tianqi Xu1, Dapeng Li1
1The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
Ultrasonics sonochemistry
|November 13, 2023
概括
这项研究引入了一种新的跨骨聚焦超声波 (tFUS) 方法,使用 perfluoropentane 纳米滴来增强空间控制的神经调节. 该技术通过精确控制微泡的形成,显著提高神经元的激活.
科学领域:
- 神经科学是一个神经科学.
- 生物医学工程 生物医学工程
- 声学物理 声学物理
背景情况:
- 非侵入性超声波神经调节 (USNM) 提供了神经疾病治疗的潜力.
- 头骨异质性和区域差异需要在USNM中改进空间控制.
- 将跨焦超声波 (tFUS) 与纳米材料相结合是大脑刺激的一个有前途的方法.
研究的目的:
- 为了提高USNM的有效性和空间可控性,使用tFUS与完全烯 (PFP) 纳米滴 (ND) 增强USNM的有效性和空间可控性.
- 开发一种用于控制神经调节的两阶段可变脉冲tFUS序列.
- 为了研究声压,纳米滴蒸发和神经元激活程度之间的关系.
主要方法:
- 开发了一种两阶段的tFUS脉冲序列,包括声滴蒸发 (ADV) 脉冲和神经调节脉冲.
- 使用PFP纳米滴 (NDs) 在ADV脉冲暴露时蒸发成微泡 (MBs).
- 调整了ADV脉冲的声压,以控制蒸发区域的大小和随后的神经元刺激的MB振荡.
- 通过c-fos+细胞表达密度量化的神经元激活在体外和体内.
主要成果:
- 与没有ADV (37.34±8.24细胞/mm2) 相比,具有ADV的PFPND显著增加了c-fos+细胞表达 (109±19细胞/mm2).
- 在体外,ADV脉冲声压为1.98 MPa和2.81 MPa,产生分别为0.146 ± 0.032 cm2和0.349 ± 0.056 cm2的蒸发区域.
- 活体内较高的声压导致更大的蒸发区域和更广泛的神经元激活.
结论:
- 开发的tFUS策略与PFP NDs和ADV一起,可以实现高效和空间可控的神经调节.
- 可控制的纳米滴蒸发是实现神经元电路精确空间准的关键.
- 这种方法为推进基于tFUS的神经调节疗法提供了有价值的框架.
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