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复星对DLD和NDUFB9的影响蒙古绵羊冷精液的减少

Yuting Chen¹, Fanhua Meng¹, Yang Liu¹

¹College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, 010018, China; Inner Mongolia Autonomous Region Key Laboratory of Biomanufacturing, Hohhot, 010018, China; Inner Mongolia Endemic Livestock Biotechnology Innovation Team, China.

|November 13, 2023

概括

复星在冷保存期间通过增加关键能量和ROS相关蛋白质来保护蒙古羊的精子,增强精子活力. 这项研究确定DLD和NDUFB9是受结影响的关键蛋白质.

科学领域:

动物科学动物科学
生殖生物学生殖生物学
蛋白质组学是指蛋白质组学.

背景情况:

蒙古羊精子的冷保存对于繁殖至关重要,但往往会降低精子的活力,改变形态.
了解精子冷保存期间的分子变化对于改善结果至关重要.

研究的目的:

为了研究复星对冷保存的蒙古公羊精子的影响.
为了确定受冷保存影响的关键蛋白质和白醇的保护作用.

主要方法:

来自蒙古公羊的精子样本分为四组进行分析:新鲜,冷而没有白醇,冷前含有白醇,解后含有白醇.
使用双重质量标签 (TMT) 标签和LC-MS/MS进行蛋白质组分析,对新鲜和冷的精子进行了分析.
生物信息学分析确定了差异丰富的蛋白质 (DAP) 和相关途径.

主要成果:

冷保存导致蛋白质表达的显著变化,发现了41个上调和48个下调的蛋白质.
参与三碳酸循环 (TCA) 和氧化酸化的关键蛋白质,如二利胺脱酶 (DLD) 和NADH脱酶1β亚复合体9 (NDUFB9),显示水平降低.
复星补充剂增加了冷保存精子中DLD和NDUFB9蛋白的表达水平.

结论:

DLD和NDUFB9被确定为关键蛋白质,有助于降低冷保存的蒙古公羊精子中的活力.
复星可以提高DLD和NDUFB9的水平,从而提高冷保存的蒙古公羊精子的活力.
这是关于蒙古公羊精子冷保存的第一个蛋白质学研究,突出了复星的保护作用.

关键词:

冰的精子,冷的精子蒙古羊是蒙古人的羊.有反应性氧物种的反应性氧物种.复星醇是一种

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