循环RNA Larp4b/miR-298-5p/Mef2c 调节由 ангиотензин II 诱导的心脏缩
Qihai Xie1,2, Xiangdong Xu3, Danqun Xiong3
1Department of Cardiology, the First Affiliated Hospital of Soochow University, Suzhou, China.
International journal of sports medicine
|November 13, 2023
概括
循环RNA Larp4b (circ_Larp4b) 通过调节microRNA-298-5p/肌细胞增强因子2轴来加剧心脏缩. 下调circ_Larp4b可能为心力衰竭提供治疗策略.
科学领域:
- 心血管生物学 心血管生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 心脏缩 (CH) 是心力衰竭进展的关键指标.
- 循环RNAs (circRNAs) 与各种人类疾病有关.
- circ_Larp4b在心肌缩中的特定作用仍未得到研究.
研究的目的:
- 为了研究circ_Larp4b在 ангиотензинII诱导的心脏缩中的功能.
- 为了阐明涉及circ_Larp4b,microRNA-298-5p和CH中的肌细胞增强因子2 (Mef2c) 的分子机制.
主要方法:
- 在HL-1细胞中使用 ангиотензинII (AngII) 治疗建立了CH的细胞模型.
- 使用qRT-PCR对circ_Larp4b,miR-298-5p和Mef2c的基因表达量化.
- 通过Western blot评估CH标志物 (ACTN2,β-MHC,ANP) 和Mef2c的蛋白质水平.
- 经过验证的分子相互作用使用双露西法酶记者和RNA拉下测试.
主要成果:
- 在 Ang II 治疗的 HL-1 细胞中,Circ_Larp4b 和 Mef2c 表达升高.
- 降低circ_Larp4b减弱的Ang II诱导的CH的调节.
- Circ_Larp4b 作为 miR-298-5p 的海绵,反过来又针对了 Mef2c.
- 在CH模型中,Mef2c的过度表达抵消了miR-298-5p对细胞大小的影响.
结论:
- 通过调节miR-298-5p/Mef2c通路,Circ_Larp4b促进心脏缩.
- Circ_Larp4b代表了治疗心脏缩和心力衰竭的潜在治疗目标.
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