甲动物心脏活性信号系统在类软体动物太平洋海
Sang Hyuck Lee1, Mi Ae Kim2, Young Chang Sohn1
1Department of Marine Bioscience, Gangneung-Wonju National University, Gangneung, Gangwon-do, Republic of Korea.
概括
这项研究描述了太平洋海中第一个甲类心脏活性 (CCAP) 信号系统,揭示了它与Ca2+/PKC和cAMP/PKA通路的联系. 这为deuterostomes中的神经S和催产素/血管压素系统提供了进化见解.
科学领域:
- 神经内分泌学神经内分泌学
- 分子进化分子进化
- 胃肠病学 胃肠病学
背景情况:
- 甲类心脏活性 (CCAP) 信号传递在关节动物中得到了很好的研究,但在lophotrochozoans中了解得很少.
- 不同动物群体中CCAP,神经S (NPS) 和催产素/血管压素 (OT/VP) 系统之间的关系尚不清楚.
- 太平洋海 (Haliotis discus hannai) 提供了一个模型来研究软体动物中的这些信号系统.
研究的目的:
- 在太平洋海中识别和描述CCAP和CCAP受体 (CCAPRs).
- 阐明 abalone CCAP 和 CCAPR 的功能关系.
- 探索CCAP,NPS和OT/VP信号通路之间的进化联系.
主要方法:
- 在Haliotis discus hannai中识别CCAP前体和CCAPR异型.
- 遗传学分析以确定CCAPRs的进化关系.
- 在HEK293细胞中进行报告器测定 (Ca2+调动,cAMP积累).
- 在体连接体-受体对接研究中.
- 在神经和外围组织中进行转录表达分析.
主要成果:
- 在Haliotis discus hannai中发现了一种CCAP前体,其中有三种CX5C型和一种CX4C型CCAP,以及两种CCAPR异型.
- Hdh-CCAPRs与原生体CCAPRs和后生体NPSRs表现出同质性,作为罗多素型G蛋白结合受体起作用.
- 鱼CCAP和昆虫CCAP通过Hdh-CCAPR诱导了Ca2+调动和cAMP积累,在对接模型中观察到特定的N终端相互作用.
- 对Hdh-CCAP前体和Hdh-CCAPR的转录主要表达在神经质中.
结论:
- 在胃类软体动物中描述了第一个CCAP信号系统,它连接了Ca2+/PKC和cAMP/PKA通路.
- 龙类CCAPRs形成了一个与关节动物CCAPRs分开的独特的进化血统.
- 这项研究为Deuterostomian NPS和OT/VP信号系统的进化起源提供了关键的见解.
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