安德罗格拉菲斯 (Andrographis paniculata) 提取物对病毒繁殖和氧化生产的抑制效率
Ittipon Siridechakorn1, Parvapan Bhattarakosol2, Thanayod Sasivimolrattana3
1Cosmetics and Natural Products Research Center, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, 65000, Thailand.
Scientific reports
|November 13, 2023
概括
安德罗格拉菲斯 (Andrographis paniculata) 提取物对A型流感 (H3N2) 和简单疹病毒-1 (HSV-1) 具有广泛的抗病毒活性. 最强效的抗病毒分数不含andrographolide衍生物,这表明了新的作用机制.
科学领域:
- 植物化学 植物化学
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 安德罗格拉菲斯 (Andrographis paniculata) 是一种药用草本,据报道具有抗病毒性质.
- 其抗病毒作用的具体机制在很大程度上仍未被描述.
研究的目的:
- 为了确定Andrographis paniculata提取物中最有效的抗病毒部分.
- 评估抗病毒活性对抗RNA (流感A H3N2) 和DNA (HSV-1) 病毒.
- 评估提取物对巨细胞中氧化产生的影响.
主要方法:
- 压缩Andrographis paniculata乙醇提取物和干粉提取物的制备.
- 在细胞感染之前和之后,用植物提取物化病毒菌株.
- 用脂聚糖激活的RAW 264.7巨细胞中氧化产生的测定.
- 高性能液体染色学 (HPLC) 和液体染色学-质谱学 (LC-MS) 分析.
主要成果:
- 几个部分 (APSP,APE,APE-2B,APE-2C) 表明直接中和HSV-1和A型 (H3N2) 流感.
- 分数APE-2A中和了流感A (H3N2),但不是HSV-1.
- 分数APE-2C显示出强烈的氧化物生产抑制,IC50为6.08μg/ml.
- 抗病毒活性在缺乏andrographolide衍生物的部分中是最高的,这些衍生物只表现出中度活性.
结论:
- 安德罗格拉菲斯 (Andrographis paniculata) 具有针对A型流感和HSV-1的广泛抗病毒活性.
- 主要的抗病毒化合物可能不是andrographolide衍生物.
- 植物提取物通过抑制氧化的产生,表现出免疫调节作用.
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