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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Jul 11, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

在单分子定位显微镜数据中检测连续的结构异质性.

Sobhan Haghparast¹, Sjoerd Stallinga², Bernd Rieger³

¹Department of Imaging Physics, Delft University of Technology, 2628 CJ, Delft, The Netherlands.

Scientific reports

|November 13, 2023

概括

此摘要是机器生成的。

这项研究引入了一种用于局部化显微镜的新方法,以检测生物颗粒中的连续结构变异. 这种方法可以通过考虑异质性来实现更准确的数据融合和重建,从而提高成像准确性.

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Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

相关实验视频

Last Updated: Jul 11, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

科学领域:

生物物理学的生物物理.
超高分辨率显微镜的使用方法
结构生物学是结构生物学.

背景情况:

定位显微镜技术如3D PAINT和STORM可以提高空间分辨率.
粒子数据的融合提高了信号噪声比,但假定结构均.
生物异质性,如形状或形状变化,可能会挑战数据的解释.

研究的目的:

开发一种没有先验知识的方法,用于检测局部化显微镜数据中的连续结构变化.
通过考虑到检测到的异质性,使显微镜数据的融合和重建更加忠实.
在实验和模拟数据集上证明方法的有效性.

主要方法:

开发了一种新的无先知的算法来识别连续的结构变化.
该方法应用于DNA原始四面体的实验3D PAINT数据和核孔综合体的2D STORM数据.
使用模拟来分析标签密度和结构变异模式对检测的影响.

主要成果:

该方法成功地检测到DNA原形四面体高度的连续变化.
使用2D STORM数据确定了核孔综合体半径的连续变化.
模拟证实了管道对标签密度和多模式结构变化的敏感性.

结论:

开发的方法有效地检测了局部化显微镜中的连续结构异质性.
考虑异质性可以提高粒子聚变和数据重建的准确性.
这种方法提供了一个更忠实的生物结构的代表,用超高分辨率显微镜成像.