在机械压缩下,LncRNA ADAMTS9-AS2 通过ADAMTS9/纤维肌菌素通过ADAMTS9调节牙周带细胞迁移
Qianyao Yu1, Kunyao Guo1, Yuhui Yang1
1Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China.
Journal of periodontal research
|November 14, 2023
概括
牙移动期间的机械力调节ADAMTS9-AS2,这是一个长非编码RNA,抑制ADAMTS9. 这促进了纤维菌素的表达和细胞外矩阵的重塑,促进了牙周带细胞的迁移.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
- 矯正牙科 矯正牙科是一種矯正牙科.
背景情况:
- 牙周带细胞 (PDLCs) 是细胞外矩阵 (ECM) 在正牙运动 (OTM) 期间重塑的关键机械传感器.
- 机械力改变ECM组件,但ECM变化与OTM细胞动态之间的联系尚不清楚.
研究的目的:
- 研究机械力如何影响PDLC形态和迁移.
- 探索ECM重塑和细胞动态之间的相互作用,包括潜在的机制.
主要方法:
- 在人体PDLCs (hPDLCs) 的实验室静态机械压缩和体内小鼠OTM模型.
- 评估hPDLC迁移使用伤口愈合和穿孔测试.
- 通过西部抹杀,免疫光学,ELISA和qRT-PCR量化ADAMTS9,纤维素 (FN) 和lncRNAADAMTS9-AS2表达;进行了ADAMTS9-AS2淘汰和RNA免疫沉.
主要成果:
- 机械压缩改变了hPDLC的形态,促进了迁移,调高了FN,降低了ADAMTS9.
- 在体内OTM模型显示了类似的蛋白质表达模式.
- 在压缩下,lncRNA ADAMTS9-AS2被上调;它的倒置抑制了hPDLC迁移,并逆转了ECM变化,与ADAMTS9直接结合.
结论:
- 机械压缩诱导ADAMTS9-AS2,它抑制ADAMTS9,促进FN表达和ECM重塑.
- 这一过程促进PDLC在OTM期间的迁移和牙周稳定.
- ADAMTS9-AS2在调节细胞动力学和ECM重塑中起着关键作用,以应对机械力.
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