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在Salvia miltiorrhiza中编码氨酸转移酶的三个SmTAT基因的表达模式和功能分析

Shuai Dong¹, Long Wang¹, Huiting Qin¹

¹Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Science, Shaanxi Normal University, Xi'an 710062, China.

International journal of molecular sciences

|November 14, 2023

概括

研究了三种Salvia miltiorrhiza铁胺转移酶 (SmTAT) 基因. 所有的SmTAT都在体内和体外活跃,其中SmTAT1的活性最高,为代谢工程提供了基础.

科学领域:

生物化学生物化学
分子生物学分子生物学
植物科学植物科学

背景情况:

氨酸转移酶 (TAT) 对于氨酸的降解至关重要.
在Salvia miltiorrhiza中存在三种TAT基因 (SmTATs),但它们的功能基本上是未知的.

研究的目的:

描述三种SmTAT基因的表达,定位和生物化学特性.
评估SmTAT蛋白质的体内和体外酶活性.

主要方法:

分析不同组织中的基因表达和对甲基斯酸盐的反应.
SmTAT蛋白的亚细胞定位.
复合SmTATs的体外酶活性测定.
通过烟草中过度表达的过渡性活体活体活性评估.

主要成果:

这三种SmTAT基因都在各种组织中表达,并对甲基斯酸盐做出反应.
SmTAT蛋白位于细胞质中.
再组合的SmTATs在体外表现出TAT活性,SmTAT1显示出对氨酸脱胺的最高催化效率.
在体内研究证实,SmTATs可将氨酸催化为4-基基酸,其中SmTAT1是最活跃的.

结论:

描述的SmTATs是参与Salvia miltiorrhiza中氨酸代谢的功能性酶.
SmTAT1是氨酸降解的关键酶,在代谢工程中对二次代谢产物产生有潜在的应用.

关键词:

萨尔维亚菌 (Salvia miltiorrhiza) 是一种多发性植物.催化活动的催化活动.过时的过度表达是暂时的.铁氨基转移酶是一种铁氨基转移酶.

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