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结合遗传和转录基因方法,识别转运器编码基因可能是类植物中可重复盐耐受性QTL的原因

Maria J Asins¹, Amanda Bullones², Veronica Raga¹

¹Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Valencia, Spain.

International journal of molecular sciences

|November 14, 2023

概括

这项研究确定了6号染色体上的关键遗传区域 (QTL),该区域控制树叶中的化物积累,这对于改善作物中盐分耐受性至关重要.

科学领域:

植物遗传学和育种.
农学和作物科学农学和作物科学
分子生物学分子生物学

背景情况:

果中的盐度压力,特别是叶子中的过度化物 (Cl-) 积累,显著降低了作物产量.
了解盐耐受性的遗传基础对于开发有弹性的类品种至关重要.

研究的目的:

为了确定与果中的叶子化物和度相关的可重复量的定量特征位点 (QTL).
确定负责化物排斥的候选基因,并提高类根茎的盐耐受性.

主要方法:

进行了两次盐耐受性实验,使用了克里奥帕特拉和Poncirus trifoliata的后代.
采用了定量特征位置 (QTL) 分析和转录组分析.
利用树clementina基因组进行基因识别和映射候选基因.

主要成果:

在染色体6上发现了一种可重复的QTL,被指定为LCl-6,可显著控制叶子Cl-度.
在LCL-6.6中发现了23个潜在的候选基因,包括酸盐输送器1/输送器家族 (NPF5.9) 和主要促进者超级家族 (MFS) 蛋白质.
细胞壁生物合成和二次代谢途径与差异性基因表达有关.

结论:

该研究提供了在LCL-6内选择有益的克利奥帕特拉普通等位基因的标记物,以改善类盐的耐受性.
这项研究有助于开发改进的类根茎,用于在盐水环境中种植.
已识别的候选基因和QTL为育种计划中的标记器辅助选择提供了目标.

关键词:

类的复制品.在Cl− 恒温状态下.这种植物是Poncirus trifoliata.在QTL分析时,我们会对QTL进行分析.塑性的可塑性塑性根的生长根的生长根的生长根茎育种根茎育种根茎育种收益率收益率收益率收益率

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