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在土豆植物中由外部应用的病毒特异性dsRNA诱导的非法典途径
Viktoriya O Samarskaya1, Nadezhda Spechenkova1, Irina Ilina1
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
International journal of molecular sciences
|November 14, 2023
概括
双链RNA (dsRNA) 用于作物保护的外部应用产生了与自然RNA干扰 (RNAi) 不同的非正规小RNA (sRNA). 这些新型sRNAs不会系统地移动,影响dSRNA作物保护策略.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 双链RNA (dsRNA) 是一种有前途的非转基因作物保护工具.
- 它的机制被认为是模仿自然RNA干扰 (RNAi) 对抗RNA病毒.
- 在外源性dSRNA应用中缺乏这种机制的直接证据.
研究的目的:
- 为了研究由土豆病毒Y (PVY) 感染和外源性dSRNA应用引起的小RNA配置文件.
- 为了比较自然感染引发的RNA干扰 (RNAi) 的生物发生途径与外部的dsRNA.
主要方法:
- 小型非编码RNA (sRNA) 的高通量测序 (HTS).
- 分析由PVY感染产生的sRNAs.
- 通过外部应用PVY dsRNA片段生成的sRNA的分析.
主要成果:
- PVY感染产生了规范的21-22 nt sRNA,这是RNAi的特征.
- 外源的PVY dsRNA产生了非正规的sRNA (约18-30nT梯子).
- 这些非正规的sRNAs显示有限的系统运动和没有过渡放大.
结论:
- 外源的dsRNA应用可能利用一个不同的sRNA生物发生路径.
- 由外部dsRNA生成的非正规sRNA在系统性作物保护方面存在局限性.
- 这些发现需要重新评估dRNA介导的作物保护机制.
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