在Oryza属中对WD40亚家族的全基因组识别,进化和表达分析
Simin Ke1, Yifei Jiang1, Mingao Zhou1
1State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.
International journal of molecular sciences
|November 14, 2023
概括
这项研究在大米中发现了383个WD40基因,揭示了它们的进化模式和在酸盐生物合成和应激耐受性方面的潜在作用. 这些发现为开发改进的水品种提供了洞察力.
科学领域:
- 植物遗传学和基因组学
- 分子进化是分子进化的过程.
- 生物化学 生物化学
背景情况:
- WD40蛋白质是关键的真核基体支架,参与植物发育,信号转导和氨酸生物合成.
- 虽然OsTTG1与大米抗生素有关,但对各种大米物种中WD40基因家族的全面进化分析缺乏.
研究的目的:
- 在九种AA基因组大米物种中对WD40-TTG1亚家族进行全基因组分析.
- 研究大米中WD40基因的进化模式,基因复制,选择压力和功能作用.
- 为潜在的农业应用确定参与安东生物合成和应激反应的候选WD40基因.
主要方法:
- 在九种AA基因组大米物种中对WD40基因进行全基因组识别和遗传学分析.
- 对大米染色体间的基因分布,对线性和细分重复的分析.
- 用于进化选择的Ka/Ks比率分析,用于功能预测的cis-acting元素分析,以及在各种治疗下表达概况 (qRT-PCR).
主要成果:
- 383个WD40基因被识别并分为四个基因组,具有不均的染色体分布和细分重复的证据.
- Ka/Ks分析表明了净化选择,而cis作用元素分析将许多基因与莉酸和光反应联系起来.
- 特定的基因 (OsiWD40-26/37/42,OsiWD40-15) 显示出与黄类/类合成的潜在联系,而OsiWD40-24则对植物激素和非生物应激反应,特别是寒冷.
结论:
- 米中的WD40亚系表现出保存的进化模式,主要是由净化选择形成的.
- 已识别的候选基因,如OsiWD40-24,在抗压力方面发挥作用,并可能参与氨酸生物合成.
- 这些发现为利用WD40基因开发有色大米,耐压力品种和形态标记提供了基础.
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