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在人类脊椎间盘中表达由质细胞系衍生的神经营养因子家族连体的表达

Tatsuya Iwasaki¹, Koji Akeda¹, Koki Kawaguchi¹

¹Department of Orthopedic Surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu City 514-8507, Mie, Japan.

International journal of molecular sciences

|November 14, 2023

概括

质细胞衍生神经营养因子 (GDNF) 家庭连体如阿尔特明 (ARTN) 和珀斯芬 (PSPN) 在退化的人类椎间盘 (IVD) 组织中被上调. 这表明它们参与了光盘性疼痛病原体的产生.

科学领域:

生物医学研究的研究.
疼痛病理生理学疼痛病理生理学
细胞生物学细胞生物学

背景情况:

质细胞系衍生神经营养因子 (GDNF) 家庭连接体 (GFLs) 参与炎症疼痛敏感化.
了解GFL参与光盘性疼痛对于开发向疗法至关重要.

研究的目的:

研究GFLs (neurturin,artemin,persephin) 和它们的受体在人类椎间盘 (IVD) 细胞和组织中的表达.
为了将GFL表达与IVD退化的早期和高级阶段相关联.

主要方法:

人体IVD细胞 (细胞核和纤维结节) 被培养.
实时PCR量化了GFL和受体 (GFRA2-GFRA4) 的mRNA表达.
免疫组织化学和西式涂抹评估了蛋白质表达.

主要成果:

在人类IVD细胞中,NRTN,ARTN,PSPN和GFRA2-GFRA4均以mRNA和蛋白质水平表达.
治疗IL-1β上调了ARTN,PSPN和GFRA2mRNA的表达.
在晚期退化阶段,ARTN,PSPN和GFRA2的表达显着更高.

结论:

GFLs (ARTN,PSPN) 和它们的受体存在于人类IVD细胞中.
ARTN,PSPN和GFRA2的表达随着IVD退化而增加.
这些发现表明,GFLs在光盘性疼痛的发病过程中起作用.

关键词:

质细胞系衍生神经变因子家族的连接体.人类人类人类人类人类人类人类椎间盘退化椎间盘退化

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