细菌性阴道炎妇女的阴道微生物群分子分析:一种新的诊断工具
Alevtina M Savicheva1, Anna A Krysanova1, Olga V Budilovskaya1
1D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductive Medicine, 199034 St. Petersburg, Russia.
International journal of molecular sciences
|November 14, 2023
概括
多重实时PCR准确地描述阴道微生物群,识别细菌性阴道炎 (BV) 中减少的乳酸菌和流行的Gardnerella vaginalis. 该测试为BV诊断提供了一个敏感和特定的替代方案.
科学领域:
- 微生物学 微生物学
- 妇科 妇科 妇科 妇科
- 分子诊断学 分子诊断学
背景情况:
- 细菌性阴道炎 (BV) 是一种常见的阴道疾病.
- 多重复合下一代测序 (NGS) 是准确的,但昂贵的.
- 多重实时PCR为阴道微生物群分析提供了更容易获得的替代方案.
研究的目的:
- 评估多重RT-PCR测试 (Femoflor) 用于在女性的阴道微生物群分析.
- 为了确定该测试对BV的诊断意义.
- 评估微生物组成,重点关注乳酸菌种和机会性病原体.
主要方法:
- 包括一个331名阴道泄漏的女性队列.
- 细菌性阴道炎 (BV) 用临床检查和纽根特标准得到证实.
- 进行多重实时PCR (Femoflor测试) 进行微生物分析.
主要成果:
- 与健康对照人群相比,BV女性的乳酸菌水平明显较低.
- 乳杆菌crispatus减少,而乳杆菌内含量在BV患者中仍然很高.
- 阴道菌 (Gardnerella vaginalis) 是 BV 的女性中最常见的机会性细菌.
- 费莫弗洛尔测试显示了对BV诊断的高灵敏度和特异性.
- 该测试确定了女性中介性阴道微生物群的感染,以及性传播疾病病原体和病毒.
结论:
- 多重实时PCR (Femoflor测试) 对于评估BV女性的阴道微生物群是有效的.
- 该测试作为一种有价值的诊断工具,有可能取代传统方法,如阿姆塞尔标准和努根特评分.
- 这种分子测试有助于识别BV,中间微生物群,以及与性病和病毒的共同感染.
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