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HSP90AB1 是传染性胃肠炎病毒感染所需的宿主因子

Daili Song¹, Yujia Zhao¹, Ying Sun¹

¹Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

International journal of molecular sciences

|November 14, 2023

概括

热冲击蛋白90 (HSP90) 有助于猪中传染性胃肠炎病毒 (TGEV) 感染. 它的抑制剂VER-82576降低病毒载量和炎症性细胞因子的产生,提供一种潜在的抗病毒策略.

科学领域:

兽医病毒学兽医病毒学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

传染性胃肠炎病毒 (TGEV) 导致严重的猪肠道疾病.
热冲击蛋白90 (HSP90) 是各种病毒感染中的关键宿主因素.
在猪冠状病毒感染中HSP90的作用需要阐明.

研究的目的:

研究HSP90在TGEV感染中的作用.
评估HSP90抑制剂VER-82576对TGEV的疗效.
分析VER-82576对TGEV诱导的炎症性细胞因子产生的影响.

主要方法:

破坏了HSP90AB1和HSP90AA1基因.
用HSP90抑制剂VER-82576和KW-2478.8进行治疗.
对TGEV M基因mRNA,N蛋白水平和病毒标位的量化.
对VER-82576.6的添加时间测定.
对促炎性细胞因子表达的分析 (IL-6,IL-12,TNF-α,CXCL10,CXCL11).

主要成果:

HSP90AB1敲除和VER-82576治疗剂量取决于TGEV M基因mRNA,N蛋白和病毒标位的减少.
HSP90AA1倒置和KW-2478治疗没有显著的影响.
在早期病毒复制过程中,VER-82576对TGEV的抑制作用最为明显.
VER-82576显著抑制了TGEV诱导的IL-6,IL-12,TNF-α,CXCL10和CXCL11的上调.

结论:

HSP90AB1作为一种增强TGEV感染的宿主因素.
HSP90抑制剂VER-82576有效地降低了TGEV感染和相关的炎症反应.
HSP90AB1代表了TGEV抗病毒药物开发的有希望的治疗标.

关键词:

在HSP90中,它是HSP90的.在TGEVV中,我们可以看到.抑制作用的抑制作用.有关炎症的细胞因子.病毒感染病毒感染

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