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混合DNA/RNA纳米结构的模板辅助组装使用分支的Oligodeoxy和Oligoribonucleotides

Alesya Fokina^1,2, Yulia Poletaeva³, Svetlana Dukova⁴

¹Faculty of Physics, Novosibirsk State University, Novosibirsk 630090, Russia.

International journal of molecular sciences

|November 14, 2023

概括

研究人员使用树突模板开发了一种新的DNA纳米结构构造方法. 这种方法成功地创造了类似C24富勒的DNA外,证明了复杂DNA自组装和纳米机器设计的新途径.

科学领域:

* 合成生物学和纳米技术
*DNA自组装和结构DNA纳米技术

背景情况:

*复杂的三维DNA纳米结构的构建对于纳米技术和合成生物学的先进应用至关重要.
*现有的方法往往面临着可扩展性和精确结构控制方面的挑战.

研究的目的:

* 开发一种模板辅助的组装方法,用于创建C24富勒烯类的双链DNA多面体外.
* 设计和合成用于DNA纳米结构制造的新型分支寡核酸构建块和树突模板.

主要方法:

* 采用超分子寡核酸树枝体作为3D模板,通过在分支结处混合siRNA和单链DNA而形成.
* 设计了一种四向分支的DNA构建块 (星色),具有自我补充的链,并结合了可光标的保护组和可切割的链接器.
* 采用固相合成构建块,然后采用模板辅助组装和传输电子显微镜进行可视化.

主要成果:

*成功合成了分支的寡氧氧和寡氧核酸构建块.
* 在DNA涂层的RNA树突模板周围组装了一个类似C24富勒伦的DNA外.
*使用传输电子显微镜可视化了所得到的纳米结构,证实了成功组装.

结论:

* 拟议的模板辅助组装方法对于构建复杂的DNA多面体外是有效的.
*分支构建块和树突模板的设计为DNA纳米结构制造提供了一个多功能策略.
*内置的可切割连接器和保护组可促进对生物介质的潜在应用进行可控拆卸.

关键词:

DNA纳米技术 DNA纳米技术有分支的寡核化物.核酸是核酸中的一种.摄影标记的保护组保护组.固相合成的固相合成方法

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