模拟微重力通过对依赖于定数感应的农业下调来降低Staphylococcus aureus中毒性因子的产生
Macauley J Green1, Ewan J Murray2, Paul Williams2
1School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
International journal of molecular sciences
|November 14, 2023
概括
微重力通过降低自诱导 (AIP) 产量来降低金黄色葡萄球菌的毒性,这种产量调节了辅助基因调节器 (agr) 系统. 这种转变在太空任务期间有利于殖民而不是病原性.
科学领域:
- 微生物学 微生物学
- 空间生物学 空间生物学
- 细菌病原体的产生
背景情况:
- 细菌污染在太空任务期间存在风险,影响机组人员的健康和设备.
- 金黄色葡萄球菌 (Staphylococcus aureus) 是一种多才多艺的细菌,充当一个共生性和机会性病原体.
- 辅助基因调节器 (agr) 系统通过定数感知来控制金色菌的毒性和殖民.
研究的目的:
- 调查低剪切模拟微重力 (LSMMG) 对S. aureus农业系统调节的影响.
- 确定自诱导 (AIP) 生产在S. aureus适应微重力的作用.
主要方法:
- 在LSMMG和地面控制条件下培养S. aureus菌株 (JE2,SH1000,USA300).
- 使用THP-1细胞系评估细菌细胞毒性并测量纤维素结合.
- 使用S. aureus agrP3::lux报告者基因融合和质谱学来量化AIP度和agr激活.
主要成果:
- 这种LSMMG条件导致S. aureus细胞毒性降低,纤维素结合增加.
- 在LSMMG下,AIP生产大幅减少,拖延了农业系统的激活.
- 微重力没有影响AgrC-AIP相互作用,但影响了AIP生成.
结论:
- 在微重力下减少AIP产量将S. aureus转向殖民化表型.
- 农业系统的毒性调节是由微重力通过AIP生成调节的.
- 了解这些机制对于减轻太空环境中的细菌风险至关重要.
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