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植物和传统甜葡萄中的基酸的组成和生物活性

Fantong Meng¹, Wantong Du¹, Yaxing Zhu¹

¹Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, Institute of Cellular and Molecular Biology, College of Life Science, Jiangsu Normal University, Xuzhou 221116, China.

Foods (Basel, Switzerland)

|November 14, 2023

概括

甜葡萄树的尖端产生高度纯化的酸 (CGA),这种化合物有效地将蔬菜与传统品种区分开来. 这种纯化的CGA在细胞系研究中也显示出强大的抗乳腺癌特性.

科学领域:

农业化学农业化学
植物化学植物化学
癌症生物学癌症生物学

背景情况:

甜葡萄的葡萄尖是丰富的基酸 (CGA) 的来源.
蔬菜和传统甜之间CGA配置的品种差异尚未得到充分证实.
研究CGA的潜在治疗应用,特别是在癌症中,越来越感兴趣.

研究的目的:

从甜葡萄树尖端提取和净化酸 (CGA).
分析和区分蔬菜和传统甜品种之间的CGA异构体概况.
为了评估纯化甜CGA (SCGA) 的抗癌活性.

主要方法:

乙醇提取和CGA从甜葡萄树尖端的连续净化.
高性能液体色谱与质谱学 (LC-MS) 结合用于异构体分析.
主要成分分析 (PCA) 和线性差异分析 (LDA) 用于品种差异化.
试验室试验评估SCGA对乳腺癌细胞系的影响 (MB231和MCF7).

主要成果:

获得了高纯度 (超过85%) 的SCGA,以4-caffeoyl酸 (4-CQA) 为主要异构体.
使用PCA和LDA,独特的CGA配置文件允许100%准确地区分蔬菜和传统的甜品种.
SCGA显著抑制了乳腺癌细胞的克隆性,迁移,入侵和肺转移.

结论:

甜葡萄树的尖端是高纯度CGA提取的可行来源.
CGA同位素分析作为可靠的生物标志物,用于区分甜品种.
纯化SCGA具有作为抗乳腺癌剂的显著潜力.

关键词:

这就是LC-MS.这是一种抗乳腺癌的药物.基酸 (CGA) 分离和净化线性差异分析 (LDA) 是一种分析方法.主要组成部分分析 (PCA)甜是一种甜.

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Glucose Transporters

Glucose Transporters

Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes: