从生牛奶中分离出来的乳酪菌株:为其在奶酪制造中作为辅助培养的资格进行选策略
Luca Bettera1, Alessia Levante1, Elena Bancalari1
1Department of Food and Drug, University of Parma, 43124 Parma, Italy.
Foods (Basel, Switzerland)
|November 14, 2023
概括
这项研究确定了用于奶酪生产的生牛奶中的非起始乳酸细菌 (NSLAB). 两个菌株,5959_Lbparacasei和5296_Lbparacasei,显示出作为长期成熟奶酪的辅助培养的希望.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 乳制品科学 乳制品科学
背景情况:
- 生牛奶和老化奶酪的微生物生态包括初始乳酸细菌 (SLAB) 和非初始乳酸细菌 (NSLAB).
- NSLAB对于奶酪香味至关重要,但它们作为辅助培养的使用需要评估除了香味之外的特征,包括对奶酪制造条件的耐受性.
- 这项研究调查了Grana Padano PDO奶酪生产中使用的原牛奶中的NSLAB.
研究的目的:
- 从自发发酵的原牛奶中分离和鉴定非起始乳酸细菌 (NSLAB).
- 评估选定的NSLAB菌株的表型特征,重点关注它们在奶酪生产中作为辅助培养的潜力.
- 确定有前途的NSLAB菌株用于生牛奶和长期成熟奶酪制造.
主要方法:
- 隔离和选择10种不同的非起始乳酸细菌 (NSLAB) 菌株,属于*Lacticaseibacillus* spp. 来自122个分离物.
- 现型特征包括在技术压力下增长表现,挥发性化合物生产,营养利用和化学耐药性.
- 在应用之前,对选择的菌株进行抗生素耐药性的评估.
主要成果:
- 确定了两个*Lacticaseibacillus paracasei*菌株 (5959_Lbparacasei和5296_Lbparacasei) 作为生牛奶和长期成熟奶酪添加剂的最佳候选者.
- 其他品种 (5293_Lbparacasei, 5649_Lbparacasei, 5780_Lbparacasei) 具有显著的芳香潜力,但耐热性较低,因此适合生产原料奶酪.
- 该研究强调了在使用辅助NSLAB培养物之前进行综合性表征的重要性,包括抗生素耐药性查.
结论:
- 精选的*Lacticaseibacillus*菌株显示出在奶酪生产中作为辅助培养的潜力,有助于微生物生态和风味特征.
- 菌株特定的特性,如耐热性和芳香化合物产生,决定了它们适用于不同类型的奶酪的适用性.
- 为了在乳制品发酵中安全有效地应用这些NSLAB附加剂,需要进一步评估,特别是关于抗生素耐药性的评估.
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