基于aptamer的光生物传感器在生物学应用中的最新进展
Minhyuk Lee1, Seonhye Shin2, Sungjee Kim1
1Department of Chemistry, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.
Molecules (Basel, Switzerland)
|November 14, 2023
概括
体是用于开发敏感光生物传感器的多功能识别工具. 本综述详细介绍了aptamer查及其用于检测疾病和污染物的用途.
科学领域:
- 生物技术和分子诊断学
- 化学生物学和传感器开发
背景情况:
- 亚胺为分子标提供高特异性和亲和力,使得定制的生物识别成为可能.
- 光感应传感器是疾病诊断和预防的宝贵工具,可以检测生物标志物,病原体和毒素.
- 它们的优点包括高灵敏度,选择性,快速检测和成本效益.
研究的目的:
- 对高特异性的aptamer选方法进行审查.
- 为了总结阿普坦酶序列和亲和关系.
- 专注于生物应用的基于aptamer的光检测传感器.
主要方法:
- 介绍阿普坦体查技术的介绍.
- 编译的阿巴美尔序列和亲和力数据.
- 光探针设计和机制的概述.
主要成果:
- 详细讨论了针对特异性的aptamer查.
- 呈现一个表格,概括了阿普坦酶序列和亲和关系.
- 探索各种基于光的检测机制.
结论:
- 对开发先进的光生物传感器来说,aptamers是至关重要的.
- 本综述提供了对光检测中阿普坦应用的全面概述.
- 突出显示了适应传感器技术的未来方向.
相关概念视频
01:19Protein Dynamics in Living Cells
2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
03:31Labeling DNA Probes
8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K