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使用Oligo-FISH对Thinopyrum中间体及其潜在原始物种的基因组分析

Fei Qi¹, Shuang Liang², Piyi Xing^1,2

¹State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China.

Plants (Basel, Switzerland)

|November 14, 2023

概括

使用分子细胞遗传学分析了中间小麦草 (IWG) 的基因组. 这项研究澄清了J和St基因组对IWG的贡献,揭示了其复杂的进化历史.

科学领域:

植物遗传学和基因组学
分子细胞遗传学分子细胞遗传学
小麦育种和遗传学

背景情况:

中间小麦草的基因组组成 (Th. 中间) 是复杂的,需要进一步阐明.
了解IWG的基因组起源对于其在作物改良中的利用至关重要.

研究的目的:

为了研究Th.的型组成. 使用分子细胞遗传学的中间体.
在中间小麦草中识别J和St基因组的祖先起源.

主要方法:

使用特定的DNA探针 (St2-80,pDb12H,pSc119.2-1, (GAA) 10,AFA-3,AFA-4,pAs1-1,Pas1-3,pAs1-4,pAs1-6) 进行了光在位杂交 (FISH).
FISH被用来分类Th.的染色体. 并为十个加入国家建立FISH类型.
进行了与相对双胞胎物种的比较分析.

主要成果:

这就是Th的J基因组. 中间物质主要是由Dasypyrum breviaristatum贡献的,而不是D. villosum.
中间小麦草具有单个J基因组,与Thinopyrum elongatum或Th.相关. 贝萨拉比克 (Bessarabicum) 是一个阿拉伯语.
圣氏基因组起源于 Pseudoroegneria 属,很可能是通过杂交事件.

结论:

这项研究澄清了中间小麦草的基因组组成,确定了关键的祖先贡献者.
在FISH模式中观察到的变异性突出显示了IWG加入内部和之间的遗传多样性.
结果为了解IWG进化及其在小麦育种计划中的潜力提供了基础知识.

关键词:

鱼类鱼类鱼类它们是三叶草 (Triticeae).进化演化演化演化演化演化演化演化基因组的构成基因组的构成型是一种型.

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