外源乳酸添加物对四种诱微藻菌株生长,光合作用性能和生物化学成分的表征
Jiawen Duan1,2,3, Chenhui Li1,2,4, Yimeng Zheng1,2,4
1State Key Laboratory of Marine Resource Utilization in the South China Sea, Hainan University, Haikou 570228, HainanChina.
Journal of applied microbiology
|November 14, 2023
概括
外源乳酸显著影响四种微藻菌株的生长和生物化学组成,影响脂质,糖和蛋白质合成. 这项研究有助于优化用于水产养殖的微藻生物质生产.
科学领域:
- 海洋生物技术 海洋生物技术
- 物理学的生理学
- 水产养殖营养水产养殖营养
背景情况:
- 微藻对于水产养殖作为诱至关重要,需要高效的生物质生产.
- 已知乳酸酸在三角的代谢调节中起作用.
- 了解影响微藻生长和组成的因素是优化它们使用的关键.
研究的目的:
- 研究外源乳酸对四种常见的诱微藻生长速度的影响.
- 评估乳酸如何影响这些微藻的光合作用效率和生物化学成分 (脂质,蛋白质,糖,颜料).
- 确定最佳的乳酸盐度,以增强微藻生物质和有价值的产品合成.
主要方法:
- 培养了四种微藻种类:三角藻 (Phaeodactylum tricornutum),藻 (Isochrysis galbana),藻 (Chaetoceros muelleri) 和藻 (Cylindrotheca fusiformis).这些种类的微藻种类包括:藻 (Phaeodactylum tricornutum),藻 (Isochrysis galbana),藻 (Chaetoceros muelleri) 和藻 (Cylindrotheca fusiformis).
- 补充培养具有不同度的外源乳酸盐.
- 测量光学密度,光合作用效率 (YII,Fv/Fm),以及量化总脂质,蛋白质,可溶糖,不可溶糖,叶绿素a和胡卜素含量.
主要成果:
- 乳酸添加增强了Isochrysis galbana的生长速度,尽管度更高降低了光合作用效率.
- 在所有测试的微藻菌株中,总脂质含量随着乳酸盐度的增加而增加.
- 观察到糖分和色素含量 (叶绿素a,胡卜素) 的显著变化,对乳酸度的反应有所不同. 在P. tricornutum. 中,蛋白质含量显著增加.
- 不同的微藻种类对生长和生化成分的乳酸补充有不同的反应.
结论:
- 外源乳酸有效调节微藻中关键成分的生长,光合作用效率和生物化学合成.
- 乳酸盐影响脂质,糖和色素的积累,为优化微藻生物质和高价值产品产量提供了潜力.
- 这些发现提供了关于乳酸盐对代谢调节的见解,这对于增强微藻在水产养殖和生物技术中的应用至关重要.
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