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Updated: Jul 11, 2025

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
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Published on: March 12, 2018

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工程三方基因编辑机器,用于高效的非病毒向基因组集成

Hangu Nam1, Keqiang Xie2, Ishita Majumdar2

  • 1Department of Bioengineering, Northeastern University, Boston, MA 02115, United States.

Research square
|November 14, 2023
PubMed
概括

研究人员开发了enGager,这是一种新的基因组编辑系统,可以提高基因敲定效率,使用圆形单链DNA (cssDNA) 捐赠者. 该系统改善了向基因整合,为治疗应用提供了比病毒载体更安全的替代方案.

关键词:
在CART-T中,我们可以使用CAR-T.这就是CRISPR/Cas9的作用.圆形单链DNA是一个圆形的单链DNA.基因组整合 基因组整合同类的定向维修.

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