一个新的外聚糖路由从淡水的Sphingomonas隔离
Alexandra G Goetsch1, Daniel Ufearo1, Griffin Keiser2
1Department of Bacteriology, University of Wisconsin - Madison, Madison, WI 53706, USA.
bioRxiv : the preprint server for biology
|November 14, 2023
概括
研究人员在Sphingomonas sp.中发现了一种新的细菌外聚糖 (EPS) 生物合成途径. LM7.7. 在 LM7.7. 这一发现促进了对细菌糖甘多样性的理解,并为设计有价值的多糖化合物提供了潜力.
科学领域:
- 微生物学 微生物学
- 葡萄糖生物学 葡萄糖生物学
- 生物化学 生物化学
背景情况:
- 细菌产生多种多糖为细胞外的装饰,复杂的生物合成途径导致各种结构和功能.
- 定义细菌糖甘生物合成途径是具有挑战性的,因为它涉及到复杂的化学成分,限制了对自然多糖制造的详细理解.
研究的目的:
- 调查细菌*Sphingomonas sp.中的外聚糖 (EPS) 生产情况. * LM7,从密歇根湖中分离出来.
- 确定和描述负责 * Sphingomonas sp. 中 EPS 生合成的基因和途径. * LM7.7 的时间.
- 阐明LM7产生的EPS的化学结构,并将其路径与已知的系统进行比较.
主要方法:
- 对 * Sphingomonas sp. 的一个转子子突变库进行选. * LM7用于改变殖民地形态,以确定参与EPS生产的基因.
- 识别编码*wzy/wzx*依赖的多糖化合物组合途径的基因群.
- 删除已识别集群中的基因,以确认它们在EPS分泌中的作用.
- 从LM7培养物中提取和分析EPS的化学成分.
主要成果:
- 在Sphingomonas sp.中发现了一种新型的*wzy/wzx*依赖的多糖化合物组装途径的基因集群. * LM7.7 的时间.
- 这一群体内的基因缺失导致粘膜表型和EPS分泌的丧失,证实了途径的参与.
- 由LM7产生的EPS由葡萄糖,银河糖和葡萄糖酸残留物线性链组成,由3和4链接连接在一起.
- 已识别的EPS通路与在较高*Alphaproteobacteria*中发现的粘合性多糖化合物不同.
结论:
- * Sphingomonas sp. 这种虫子是什么? * LM7 具有独特的 * wzy / wzx * -依赖于外聚糖生物合成的途径.
- 描述完整的细菌多糖生物合成途径为了解甘氨酸多样性提供了基础.
- 这项研究为具有可取性质的新型多糖体的潜在工程开辟了道路.
相关概念视频
Formation of Lipopolysaccharides
21
Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
21
Oligosaccharide Assembly
2.9K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.9K
Gene Regulation During Sporulation
20
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
20
Bacterial Phylum Bacteroidota
24
The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...
24
Peptidoglycan Synthesis
22
Structure of PeptidoglycanPeptidoglycan is a vital structural component of the bacterial cell wall, providing mechanical strength and shape to the cell. It consists of repeating units of two sugars—N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM)—linked by β-1,4 glycosidic bonds. These sugar chains are cross-linked by short peptide chains, forming a mesh-like polymer that surrounds the bacterial plasma membrane.Cytoplasmic Phase – Precursor SynthesisPeptidoglycan...
22


