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Updated: Jul 11, 2025

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block
06:10

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

Published on: April 8, 2021

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理论框架和实验解决方案的空气-水接口吸附问题在冷EM.

Joon S Kang, Xueting Zhou, Yun-Tao Liu

    bioRxiv : the preprint server for biology
    |November 14, 2023
    PubMed
    概括

    低温电子显微镜 (cryoEM) 由于空气-水界面 (AWI) 吸附而导致的粒子损失. 这项研究解释了粒子迁移到AWI,并证明表面活性剂是高分辨率冷EM的实际解决方案.

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    Optimizing Sample Preparation for Cryogenic Electron Microscopy06:32

    Optimizing Sample Preparation for Cryogenic Electron Microscopy

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    相关概念视频

    Cryo-electron Microscopy01:28

    Cryo-electron Microscopy

    Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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